Optimized production of 2,3-butanediol by a lactate dehydrogenase-deficient mutant of Klebsiella pneumoniae

• Published in: Biotechnology and bioprocess engineering Vol. 18; no. 6; pp. 1210 - 1215
• Main Authors: Lee, Sung-Mok, Oh, Baek-Rock, Park, Jang Min, Yu, Anna, Heo, Sun-Yeon, Hong, Won-Kyung, Seo, Jeong-Woo, Kim, Chul Ho
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2013; Springer Nature B.V; 한국생물공학회
• Subjects: Agricultural biotechnology; Biodiesel fuels; Biotechnology; Carbon; Carbon sources; Chemistry; Chemistry and Materials Science; Chromatography; Dehydrogenase; Dehydrogenases; Ethanol; Fermentation; Glucose; Industrial and Production Engineering; Klebsiella pneumoniae; Metabolism; Metabolites; Microorganisms; Productivity; Research Paper; Statistical significance; Studies; Sugarcane; Syrups & sweeteners; Variance analysis; 생물공학; South Korea; response surface methodology; lactate dehydrogenasedeficient mutant; 2,3-butanediol; sugarcane molasses
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-013-0396-z

To obtain high-yield production of 2,3-butanediol (2,3-BD) from glucose, we optimized the culture conditions for a lactate dehydrogenase-deficient mutant ( ΔldhA ) of Klebsiella pneumoniae using response surface methodology. 2,3-BD production was successfully improved by optimizing pH (5.6), aeration (3.50 vvm) and concentration of corn steep liquor (45.0 mL/L) as a nitrogen source, resulting in a maximum level of 2,3-BD production of 148.8 g/L and productivity of 2.48 g/L/h. 2,3-BD was also obtained with high concentration (76.24 g/L) and productivity (2.31 g/L/h) from the K. pneumoniae mutant strain using sugarcane molasses as a carbon source.