Development of recombinant subunit vaccine targeting InvH protein of Salmonella Typhimurium and evaluation of its immunoprotective efficacy against salmonellosis
Salmonella Typhimurium is the most prevalent non-host specific Salmonella serovars and a major concern for both human and animal health systems worldwide contributing to significant economic loss. Type 3 secretion system (T3SS) of Salmonella plays an important role in bacterial adherence and entry i...
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Published in | Brazilian journal of microbiology Vol. 54; no. 4; pp. 3257 - 3264 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Cham
Springer International Publishing
01.12.2023
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Salmonella
Typhimurium is the most prevalent non-host specific
Salmonella
serovars and a major concern for both human and animal health systems worldwide contributing to significant economic loss. Type 3 secretion system (T3SS) of
Salmonella
plays an important role in bacterial adherence and entry into the host epithelial cells. The product of invH gene of
Salmonella
is an important component of the needle complex of the type 3 secretion system. Hence, the present study was undertaken to clone and express the 15 kDa InvH surface protein of
Salmonella
Typhimurium in an
E. coli
host and to evaluate its immune potency in mice. The purified recombinant InvH (r-InvH) protein provoked a significant (
p
< 0.01) rise in IgG in the inoculated mice. The immunized mice were completely (100%) protected against the challenge dose of 107.5 LD50, while protection against challenge with the same dose of heterologous serovars was 90%. The bacterin-vaccinated group showed homologous protection of 60% against all three serovars. Findings in this study suggest the potential of the r-InvH protein of
S
. Typhimurium as an effective vaccine candidate against
Salmonella
infections. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Responsible Editor: Roxane M Piazza |
ISSN: | 1517-8382 1678-4405 1678-4405 |
DOI: | 10.1007/s42770-023-01136-6 |