In vitro propagation, microtuberization, and molecular characterization of three potato cultivars

• Published in: Biologia plantarum Vol. 61; no. 3; pp. 427 - 437
• Main Authors: Salem, J., Hassanein, A. M.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.09.2017; Springer Nature B.V; Institute of Experimental Botany of the Czech Academy of Sciences
• Subjects: auxins; Biomedical and Life Sciences; Butyric acid; Carbon; Chemical vapor synthesis; Cultivars; Culture media; cytokinins; Dichlorophenoxyacetic acid; Fructose; gene expression; Gibberellic acid; Glucose; In vitro methods and tests; Indole-3-butyric acid; Kinetin; Life Sciences; Micropropagation; Naphthaleneacetic acid; Original Paper; Plant Sciences; Potatoes; Propagation; rapd; Shoots; solanum tuberosum; Sucrose; Sugar; Tubers; cytokinins; auxins; micropropagation; RAPD; gene expression
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/s10535-017-0715-x

Sprouts of potato tubers were excised from the three potato cultivars Agria, Hermes, and Spunta, sterilized and subjected to shoot formation and propagation on Murashige and Skoog (MS) medium supplemented with 1 mg dm -3 6-benzylaminopurine (BAP) + 0.5 mg dm -3 gibberellic acid. Shoots were rooted on MS medium supplemented with 1 mg dm -3 indole-3-butyric acid. To increase shoot vigour prior tuber formation, shoots were subcultured on MS medium supplemented with 0.56 mg dm -3 BAP, 0.11 mg dm -3 2,4-dichlorophenoxyacetic acid, and 0.96 mg dm -3 naphthaleneacetic acid. Under dark, microtuberization on MS media supplemented with 4 mg dm -3 of both BAP and kinetin was better than 4 mg dm -3 BAP alone, where they induced higher number of microtubers per shoot and/or the percentage of shoots that formed microtubers. The highest frequency of microtuber formation was achieved when sucrose at high concentration (8 %) was used as carbon source in culture media. Glucose ranked at the second position whereas fructose reduced the microtuber formation frequency when it was used alone or in combination with glucose. Under the applied culture conditions, cvs. Agria and Hermes showed better micropropagation and microtuberization in comparison to cv. Spunta. In addition, isozyme and RAPD techniques revealed that Agria and Hermes are closer to each other when compared with the third cultivar.