Re-characterization of an extrachromosomal circular plasmid in the pathogenic Leptospira interrogans serovar Lai strain 56601
In China, Leptospira interrogans serovar Lai strain 56601 （str.56601） is one of main pathogenic strains that cause severe leptospirosis in both human and animals. The genome of this organism was completely sequenced in 2003. However, in 2011, we identified and corrected some assembly errors in the s...
Saved in:
| Published in | Acta biochimica et biophysica Sinica Vol. 46; no. 7; pp. 605 - 611 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
China
01.07.2014
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-9145 1745-7270 1745-7270 |
| DOI | 10.1093/abbs/gmu033 |
Cover
Loading…
| Abstract | In China, Leptospira interrogans serovar Lai strain 56601 （str.56601） is one of main pathogenic strains that cause severe leptospirosis in both human and animals. The genome of this organism was completely sequenced in 2003. However, in 2011, we identified and corrected some assembly errors in the str.56601 genome due to the repeat sequences widely distributed in the Leptospira genome. In this study, we re-analyzed the previously reported mobile, phage-related genomic island in the chromosome and rectified detailed sequence information in both the plasmid and chromosome using various experimental methods. The presence of a separate circular extrachromosomal plasmid was also confirmed, and its location in the genomi~ region was determined relative to the genomic island reported in L. interrogans serovar Lai by a combination of pulsed-field gel electrophoresis -based and plasmid extraction-based Southern blot analysis. This report confirmed that the sep arate extrachromosomal circular plasmid is not integrated into the chromosome of. interrogans str.56601 and markedly improved our understanding of the genomic organization, evolution, and pathogenesis of L. interrogans. In particular, characterization of this extrachromosomal cir cular plasmid will contribute to the development of genetic manipulation systems in pathogenic Leptospira species. |
|---|---|
| AbstractList | In China, Leptospira interrogans serovar Lai strain 56601 (str.56601) is one of main pathogenic strains that cause severe leptospirosis in both human and animals. The genome of this organism was completely sequenced in 2003. However, in 2011, we identified and corrected some assembly errors in the str.56601 genome due to the repeat sequences widely distributed in the Leptospira genome. In this study, we re-analyzed the previously reported mobile, phage-related genomic island in the chromosome and rectified detailed sequence information in both the plasmid and chromosome using various experimental methods. The presence of a separate circular extrachromosomal plasmid was also confirmed, and its location in the genomic region was determined relative to the genomic island reported in L. interrogans serovar Lai by a combination of pulsed-field gel electrophoresis -based and plasmid extraction-based Southern blot analysis. This report confirmed that the separate extrachromosomal circular plasmid is not integrated into the chromosome of L. interrogans str.56601 and markedly improved our understanding of the genomic organization, evolution, and pathogenesis of L. interrogans. In particular, characterization of this extrachromosomal circular plasmid will contribute to the development of genetic manipulation systems in pathogenic Leptospira species. In China, Leptospira interrogans serovar Lai strain 56601 (str.56601) is one of main pathogenic strains that cause severe leptospirosis in both human and animals. The genome of this organism was completely sequenced in 2003. However, in 2011, we identified and corrected some assembly errors in the str.56601 genome due to the repeat sequences widely distributed in the Leptospira genome. In this study, we re-analyzed the previously reported mobile, phage-related genomic island in the chromosome and rectified detailed sequence information in both the plasmid and chromosome using various experimental methods. The presence of a separate circular extrachromosomal plasmid was also confirmed, and its location in the genomic region was determined relative to the genomic island reported in L. interrogans serovar Lai by a combination of pulsed-field gel electrophoresis -based and plasmid extraction-based Southern blot analysis. This report confirmed that the separate extrachromosomal circular plasmid is not integrated into the chromosome of L. interrogans str.56601 and markedly improved our understanding of the genomic organization, evolution, and pathogenesis of L. interrogans. In particular, characterization of this extrachromosomal circular plasmid will contribute to the development of genetic manipulation systems in pathogenic Leptospira species.In China, Leptospira interrogans serovar Lai strain 56601 (str.56601) is one of main pathogenic strains that cause severe leptospirosis in both human and animals. The genome of this organism was completely sequenced in 2003. However, in 2011, we identified and corrected some assembly errors in the str.56601 genome due to the repeat sequences widely distributed in the Leptospira genome. In this study, we re-analyzed the previously reported mobile, phage-related genomic island in the chromosome and rectified detailed sequence information in both the plasmid and chromosome using various experimental methods. The presence of a separate circular extrachromosomal plasmid was also confirmed, and its location in the genomic region was determined relative to the genomic island reported in L. interrogans serovar Lai by a combination of pulsed-field gel electrophoresis -based and plasmid extraction-based Southern blot analysis. This report confirmed that the separate extrachromosomal circular plasmid is not integrated into the chromosome of L. interrogans str.56601 and markedly improved our understanding of the genomic organization, evolution, and pathogenesis of L. interrogans. In particular, characterization of this extrachromosomal circular plasmid will contribute to the development of genetic manipulation systems in pathogenic Leptospira species. In China, Leptospira interrogans serovar Lai strain 56601 （str.56601） is one of main pathogenic strains that cause severe leptospirosis in both human and animals. The genome of this organism was completely sequenced in 2003. However, in 2011, we identified and corrected some assembly errors in the str.56601 genome due to the repeat sequences widely distributed in the Leptospira genome. In this study, we re-analyzed the previously reported mobile, phage-related genomic island in the chromosome and rectified detailed sequence information in both the plasmid and chromosome using various experimental methods. The presence of a separate circular extrachromosomal plasmid was also confirmed, and its location in the genomi~ region was determined relative to the genomic island reported in L. interrogans serovar Lai by a combination of pulsed-field gel electrophoresis -based and plasmid extraction-based Southern blot analysis. This report confirmed that the sep arate extrachromosomal circular plasmid is not integrated into the chromosome of. interrogans str.56601 and markedly improved our understanding of the genomic organization, evolution, and pathogenesis of L. interrogans. In particular, characterization of this extrachromosomal cir cular plasmid will contribute to the development of genetic manipulation systems in pathogenic Leptospira species. |
| Author | Lili Huang Weinan Zhui Ping He Yan Zhang Xuran Zhuang Guoping Zhao Xiaokui Guo Jinhong Qin Yongzhang Zhu |
| AuthorAffiliation | Department of Microbiology and Immunology, Institute of Medical Science, Shanghai Jiao Tong University School of Medicine, 280 SouthChongqing Road, Shanghai 200025, China CAS-Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, ChineseAcademy of Sciences, Shanghai 200032, China |
| Author_xml | – sequence: 1 givenname: Lili surname: Huang fullname: Huang, Lili – sequence: 2 givenname: Weinan surname: Zhu fullname: Zhu, Weinan – sequence: 3 givenname: Ping surname: He fullname: He, Ping – sequence: 4 givenname: Yan surname: Zhang fullname: Zhang, Yan – sequence: 5 givenname: Xuran surname: Zhuang fullname: Zhuang, Xuran – sequence: 6 givenname: Guoping surname: Zhao fullname: Zhao, Guoping – sequence: 7 givenname: Xiaokui surname: Guo fullname: Guo, Xiaokui – sequence: 8 givenname: Jinhong surname: Qin fullname: Qin, Jinhong – sequence: 9 givenname: Yongzhang surname: Zhu fullname: Zhu, Yongzhang |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/24874103$$D View this record in MEDLINE/PubMed |
| BookMark | eNp1kc2L1TAUxYOMOB-6ci_B1YB0JrdJ03YpgzMKDwZE1-U2vW0jbdJJWlFh_nfzfM9ZCK5uuPd3TuCcc3bivCPGXoO4AlHLa2zbeD3Mm5DyGTuDUhVZmZfiJL11mWc1qOKUncf4TQipNYgX7DRXValAyDP2-JkyM2JAs1Kwv3C13nHfc3ScfqxpPQY_--hnnLixwWwTBr5MGGfbcev4OhJfcB39QM4avqNl9XGxAdMxOQY_oIs8UvDfk3CHlsfkmoSF1gJesuc9TpFeHecF-3r74cvNx2x3f_fp5v0uM7LK16xEJduc2lL0sqihrkzVkexN0aEGqbTuiqJVALqrSlNrEkgVAGrSOh1bJS_Y5cF3Cf5ho7g2s42Gpgkd-S02UCiQlcxLSOibI7q1M3XNEuyM4WfzN7IEvDsAJvgYA_VPCIhmX0izL6Q5FJJo-Ic2dv2T8j6G6T-at0fN6N3wYN3w9IXWeaoTcil_A4UynNE |
| CitedBy_id | crossref_primary_10_1038_s41598_018_29983_6 crossref_primary_10_1128_genomeA_00882_15 crossref_primary_10_1371_journal_pntd_0004220 crossref_primary_10_3389_fmicb_2021_731937 crossref_primary_10_1371_journal_ppat_1012161 |
| Cites_doi | 10.1186/1297-9716-44-49 10.1016/j.vetmic.2011.02.055 10.1016/j.vetmic.2009.03.012 10.1186/1471-2164-11-729 10.1128/IAI.01067-06 10.1371/journal.pgen.1003539 10.1038/cr.2011.46 10.1016/S0923-2508(99)00125-4 10.1038/nrmicro2208 10.1038/cr.2009.127 10.1590/S0100-879X2004000400003 10.1016/j.micinf.2008.12.007 10.1186/1471-2164-7-293 10.1111/j.1365-2958.1994.tb01074.x 10.1007/s12038-008-0074-z 10.1128/JB.186.7.2164-2172.2004 10.1038/nature01597 10.1186/1471-2164-8-204 10.1089/omi.2013.0043 10.2217/fmb.10.102 10.1186/1471-2180-6-51 |
| ContentType | Journal Article |
| Copyright | The Author 2014. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. |
| Copyright_xml | – notice: The Author 2014. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. |
| DBID | 2RA 92L CQIGP W94 WU4 ~WA AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1093/abbs/gmu033 |
| DatabaseName | 维普_期刊 中文科技期刊数据库-CALIS站点 维普中文期刊数据库 中文科技期刊数据库-自然科学 中文科技期刊数据库-自然科学-生物科学 中文科技期刊数据库- 镜像站点 CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| DocumentTitleAlternate | Re-characterization of an extrachromosomal circular plasmid in the pathogenic Leptospira interrogans serovar Lai strain 56601 |
| EISSN | 1745-7270 |
| EndPage | 611 |
| ExternalDocumentID | 24874103 10_1093_abbs_gmu033 662145123 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | --- -01 -0A -SA -S~ .2P .3N .I3 0R~ 188 1OC 1TH 23M 2RA 31~ 36B 4.4 48X 53G 5GY 5VR 5VS 5XA 5XB 5XL 6J9 70D 8-1 8RM 92L 92M 9D9 9DA AABJS AABMN AAESY AAIJN AAIMJ AAIYJ AAJKP AAJQQ AAMDB AAMVS AAOGV AAPQZ AAPXW AAUQX AAVAP AAVLN ABEUO ABIXL ABNKS ABPTD ABQLI ABZBJ ACGFO ACGFS ACUFI ACXQS ACYTK ADBBV ADEIU ADEYI ADFTL ADGZP ADHKW ADHZD ADOCK ADORX ADQLU ADRIX ADRTK ADYVW ADZTZ ADZXQ AECKG AEGPL AEGXH AEJOX AEKKA AEKSI AELWJ AEMDU AENEX AENZO AEPUE AETBJ AEWNT AFBPY AFFZL AFIYH AFOFC AFUIB AFXEN AFZJQ AGINJ AGKEF AGSYK AHXPO AIJHB AIKOY AJAOE AJEUX AKHUL AKWXX ALMA_UNASSIGNED_HOLDINGS ALTZX ALUQC APIBT APWMN ARIXL ARQIP AUCZF AXUDD AYOIW AZFZN AZQFJ AZVOD BAWUL BAYMD BCRHZ BEYMZ BFHJK BHONS BQDIO BQUQU BSWAC BTQHN BYORX CAG CAJEA CAJUS CASEJ CCEZO CCVFK CDBKE CHBEP CO8 COF CQIGP CS3 CW9 CZ4 DAKXR DCZOG DIK DILTD DPORF DPPUQ D~K E3Z EBS EE~ EJD EMB EMOBN EX3 F5P F9B FA0 FHSFR FLIZI FLUFQ FOEOM FQBLK GAUVT GJXCC GROUPED_DOAJ GX1 H5~ HAR HH5 HW0 HZ~ IOX J21 JUIAU K97 KOP KSI KSN M-Z M49 N9A NGC NLBLG NMDNZ NU- O9- OAWHX ODMLO OJQWA OJZSN OK1 OVD PAFKI PEELM Q-- Q-0 Q1. Q5Y R-A RD5 ROX RPM RT1 RUSNO RW1 RXO S.. SV3 T8Q TEORI TJP TLC U1F U1G U5A U5K UZ3 UZ4 W2D W94 WOW WU4 X7H YAYTL YKOAZ YXANX ~91 ~WA AAYXX ABEJV ABGNP ABXVV CITATION H13 JXSIZ CGR CUY CVF ECM EIF NPM 7X8 |
| ID | FETCH-LOGICAL-c382t-7a43b2eb70f359198c8de3fc5da613466d55b4116d87c96e0ae811a6e66466b43 |
| ISSN | 1672-9145 1745-7270 |
| IngestDate | Fri Jul 11 09:45:24 EDT 2025 Mon Jul 21 06:01:23 EDT 2025 Tue Jul 01 04:20:31 EDT 2025 Thu Apr 24 23:03:17 EDT 2025 Wed Feb 14 10:20:49 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 7 |
| Keywords | extrachromosomal circular plasmid Leptospira interrogans genomic island |
| Language | English |
| License | The Author 2014. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c382t-7a43b2eb70f359198c8de3fc5da613466d55b4116d87c96e0ae811a6e66466b43 |
| Notes | 31-1940/Q genomic island; Leptospira interrogans;extrachromosomal circular plasmid Lili Huang, Weinan Zhu, Ping He,Yan Zhang, Xuran Zhuang, Guoping Zhao, Xiaokui Guoa, Jinhong Qin, and Yongzhang Zhu. 1.Department of Microbiology and Immunology, Institute of Medical Science, Shanghai Jiao Tong University School of Medicine, 280 South Chongqing Road, Shanghai 200025, China 2CAS-Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China tThese authors contributed equally to this work. ＊Correspondence address. Tel： ＋86-21-63846590-778017; Fax： ＋86-21-64453285; E-mail： yzhzhu@hotmail.com （Y.Z.）/jinhongqin@sjtu.edu.cn （J.Q.） In China, Leptospira interrogans serovar Lai strain 56601 （str.56601） is one of main pathogenic strains that cause severe leptospirosis in both human and animals. The genome of this organism was completely sequenced in 2003. However, in 2011, we identified and corrected some assembly errors in the str.56601 genome due to the repeat sequences widely distributed in the Leptospira genome. In this study, we re-analyzed the previously reported mobile, phage-related genomic island in the chromosome and rectified detailed sequence information in both the plasmid and chromosome using various experimental methods. The presence of a separate circular extrachromosomal plasmid was also confirmed, and its location in the genomi~ region was determined relative to the genomic island reported in L. interrogans serovar Lai by a combination of pulsed-field gel electrophoresis -based and plasmid extraction-based Southern blot analysis. This report confirmed that the sep arate extrachromosomal circular plasmid is not integrated into the chromosome of. interrogans str.56601 and markedly improved our understanding of the genomic organization, evolution, and pathogenesis of L. interrogans. In particular, characterization of this extrachromosomal cir cular plasmid will contribute to the development of genetic manipulation systems in pathogenic Leptospira species. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 24874103 |
| PQID | 1541383271 |
| PQPubID | 23479 |
| PageCount | 7 |
| ParticipantIDs | proquest_miscellaneous_1541383271 pubmed_primary_24874103 crossref_primary_10_1093_abbs_gmu033 crossref_citationtrail_10_1093_abbs_gmu033 chongqing_primary_662145123 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2014-07-01 |
| PublicationDateYYYYMMDD | 2014-07-01 |
| PublicationDate_xml | – month: 07 year: 2014 text: 2014-07-01 day: 01 |
| PublicationDecade | 2010 |
| PublicationPlace | China |
| PublicationPlace_xml | – name: China |
| PublicationTitle | Acta biochimica et biophysica Sinica |
| PublicationTitleAlternate | Acta Biochimica et Biophysica Sinica |
| PublicationYear | 2014 |
| References | Zhong Y (null) 2011; 21 Lim VK (null) 2011; 33 Qin JH (null) 2006; 6 Zou QH (null) 2010; 11 Bhattacharya P (null) 1999; 37 Hill CW (null) 1999; 150 Ko AI (null) 2009; 7 Adler B (null) 2011; 153 Cao XJ (null) 2010; 20 Bourhy P (null) 2007; 75 Nascimento AL (null) 2004; 37 Xue F (null) 2009; 11 null Vijayachari P (null) 2008; 33 He P (null) 2007; 8 Yang HL (null) 2006; 7 Ren SX (null) 2003; 422 Nascimento AL (null) 2004; 186 Hill CW (null) 1994; 12 Matos RC (null) 2013; 9 Zeng L (null) 2013; 17 Vannucci FA (null) 2013; 44 Adler B (null) 2010; 140 Evangelista KV (null) 2010; 5 |
| References_xml | – volume: 44 start-page: 49 year: 2013 ident: null publication-title: Vet Res doi: 10.1186/1297-9716-44-49 – volume: 33 start-page: 1 year: 2011 ident: null publication-title: Malays J Pathol – volume: 153 start-page: 73 year: 2011 ident: null publication-title: Vet Microbiol doi: 10.1016/j.vetmic.2011.02.055 – volume: 140 start-page: 287 year: 2010 ident: null publication-title: Vet Microbiol doi: 10.1016/j.vetmic.2009.03.012 – volume: 11 start-page: 729 year: 2010 ident: null publication-title: BMC Genomics doi: 10.1186/1471-2164-11-729 – volume: 75 start-page: 677 year: 2007 ident: null publication-title: Infect Immun doi: 10.1128/IAI.01067-06 – volume: 9 start-page: e1003539 year: 2013 ident: null publication-title: PLoS Genet doi: 10.1371/journal.pgen.1003539 – volume: 21 start-page: 1210 year: 2011 ident: null publication-title: Cell Res doi: 10.1038/cr.2011.46 – volume: 150 start-page: 665 year: 1999 ident: null publication-title: Res Microbiol doi: 10.1016/S0923-2508(99)00125-4 – volume: 7 start-page: 736 year: 2009 ident: null publication-title: Nat Rev Microbiol doi: 10.1038/nrmicro2208 – volume: 20 start-page: 197 year: 2010 ident: null publication-title: Cell Res doi: 10.1038/cr.2009.127 – volume: 37 start-page: 459 year: 2004 ident: null publication-title: Braz J Med Biol Res doi: 10.1590/S0100-879X2004000400003 – volume: 11 start-page: 328 year: 2009 ident: null publication-title: Microbes Infect doi: 10.1016/j.micinf.2008.12.007 – volume: 7 start-page: 293 year: 2006 ident: null publication-title: BMC Genomics doi: 10.1186/1471-2164-7-293 – volume: 12 start-page: 865 year: 1994 ident: null publication-title: Mol Microbiol doi: 10.1111/j.1365-2958.1994.tb01074.x – volume: 33 start-page: 557 year: 2008 ident: null publication-title: J Biosci doi: 10.1007/s12038-008-0074-z – volume: 186 start-page: 2164 year: 2004 ident: null publication-title: J Bacteriol doi: 10.1128/JB.186.7.2164-2172.2004 – volume: 422 start-page: 888 year: 2003 ident: null publication-title: Nature doi: 10.1038/nature01597 – ident: null – volume: 8 start-page: 204 year: 2007 ident: null publication-title: BMC Genomics doi: 10.1186/1471-2164-8-204 – volume: 17 start-page: 527 year: 2013 ident: null publication-title: OMICS doi: 10.1089/omi.2013.0043 – volume: 37 start-page: 1022 year: 1999 ident: null publication-title: Indian J Exp Biol – volume: 5 start-page: 1413 year: 2010 ident: null publication-title: Future Microbiol doi: 10.2217/fmb.10.102 – volume: 6 start-page: 51 year: 2006 ident: null publication-title: BMC Microbiol doi: 10.1186/1471-2180-6-51 |
| SSID | ssj0036610 |
| Score | 2.0353673 |
| Snippet | In China, Leptospira interrogans serovar Lai strain 56601 （str.56601） is one of main pathogenic strains that cause severe leptospirosis in both human and... In China, Leptospira interrogans serovar Lai strain 56601 (str.56601) is one of main pathogenic strains that cause severe leptospirosis in both human and... |
| SourceID | proquest pubmed crossref chongqing |
| SourceType | Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 605 |
| SubjectTerms | Base Sequence Chromosomes, Bacterial DNA Primers Leptospira interrogans - genetics Leptospira interrogans - pathogenicity Plasmids 基因组结构 染色体 环状 脉冲场凝胶电泳 致病性 质粒 鉴定 钩端螺旋体病 |
| Title | Re-characterization of an extrachromosomal circular plasmid in the pathogenic Leptospira interrogans serovar Lai strain 56601 |
| URI | http://lib.cqvip.com/qk/90160X/201407/662145123.html https://www.ncbi.nlm.nih.gov/pubmed/24874103 https://www.proquest.com/docview/1541383271 |
| Volume | 46 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3_i9MwFA_zRPAX8fvNU4lwPzl6tk2arD-K3DhknKAbzJ9KkqZb4dbOrRU88H_3pUnbnU44_aVsWZJ2eZ-8vLy-9wlCp3GoFaGZ8kLOuUepJJ6MY-lJBVNPkyzKZBNtccku5vTjIloMBvV-dkklz9T1wbyS_5EqlIFcTZbsP0i26xQK4DPIF64gYbjeSsaftac6wuXrzvgThri_guKVibXblWvDAZJvbcTpBszldZ628Y3mROISbpCr0VRvqrJ58d6QSGy35RLWsRH8k_I7NJyK3GSWCGgIxpnzDbT8taoSI5mXapUb-oGRrsw36zURoy9N8mUPIOeinuZXee-3rptoP20Cc3r_bGPjtovrvnv7q6vl_BUB7WJbWxXLqTlA1x4XcqYPlDm97FyTFn98T8myJlH7T-VvibGElDADJst17VuKjZsk25efksl8Ok1m54vZHXQXIBoGrZPHLuAETBaXR2sfyqV1QvfmgLfdO9u1IeNYlcXyG4zCTaPmLzuVxmKZPUQP3FYDv7e4eYQGuniM7tnDR388QT8PoAeXGRYF_h09uEUPdujBeYEBPbhHD-7Rg_fQgx16MKAHW_TgBj1P0XxyPvtw4bnDODxFxmHlcUGJDLXkfkaiOIjHapzCXFZRKsAipIylUSRpELB0zFXMtC_0OAgE04zBj5KSZ-ioKAt9jHCWRr4vJOU05TQ0ZAYh4bCvBcUQxrCiDNFJN7LJxpKuJIwZTn2ws4bobTvWiXI89ubxrxIbT0ESI6TECmmITrvKbU8Hq71phZaAejXvzEShy3qXwA4jILDq8WCInltpdh2FsNmngU9e3KL1CbrfT4aX6Kja1voVmLOVfN2A7xdL_qa9 |
| linkProvider | National Library of Medicine |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Re-characterization+of+an+extrachromosomal+circular+plasmid+in+the+pathogenic+Leptospira+interrogans+serovar+Lai+strain+56601&rft.jtitle=Acta+biochimica+et+biophysica+Sinica&rft.au=Huang%2C+Lili&rft.au=Zhu%2C+Weinan&rft.au=He%2C+Ping&rft.au=Zhang%2C+Yan&rft.date=2014-07-01&rft.issn=1745-7270&rft.eissn=1745-7270&rft.volume=46&rft.issue=7&rft.spage=605&rft_id=info:doi/10.1093%2Fabbs%2Fgmu033&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F90160X%2F90160X.jpg |