Re-characterization of an extrachromosomal circular plasmid in the pathogenic Leptospira interrogans serovar Lai strain 56601

In China, Leptospira interrogans serovar Lai strain 56601 （str.56601） is one of main pathogenic strains that cause severe leptospirosis in both human and animals. The genome of this organism was completely sequenced in 2003. However, in 2011, we identified and corrected some assembly errors in the s...

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Published inActa biochimica et biophysica Sinica Vol. 46; no. 7; pp. 605 - 611
Main Authors Huang, Lili, Zhu, Weinan, He, Ping, Zhang, Yan, Zhuang, Xuran, Zhao, Guoping, Guo, Xiaokui, Qin, Jinhong, Zhu, Yongzhang
Format Journal Article
LanguageEnglish
Published China 01.07.2014
Subjects
Base Sequence
Chromosomes, Bacterial
DNA Primers
Leptospira interrogans - genetics
Leptospira interrogans - pathogenicity
Plasmids
基因组结构
染色体
环状
脉冲场凝胶电泳
致病性
质粒
鉴定
钩端螺旋体病
extrachromosomal circular plasmid
Leptospira interrogans
genomic island
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Summary:In China, Leptospira interrogans serovar Lai strain 56601 （str.56601） is one of main pathogenic strains that cause severe leptospirosis in both human and animals. The genome of this organism was completely sequenced in 2003. However, in 2011, we identified and corrected some assembly errors in the str.56601 genome due to the repeat sequences widely distributed in the Leptospira genome. In this study, we re-analyzed the previously reported mobile, phage-related genomic island in the chromosome and rectified detailed sequence information in both the plasmid and chromosome using various experimental methods. The presence of a separate circular extrachromosomal plasmid was also confirmed, and its location in the genomi~ region was determined relative to the genomic island reported in L. interrogans serovar Lai by a combination of pulsed-field gel electrophoresis -based and plasmid extraction-based Southern blot analysis. This report confirmed that the sep arate extrachromosomal circular plasmid is not integrated into the chromosome of. interrogans str.56601 and markedly improved our understanding of the genomic organization, evolution, and pathogenesis of L. interrogans. In particular, characterization of this extrachromosomal cir cular plasmid will contribute to the development of genetic manipulation systems in pathogenic Leptospira species.
Bibliography:31-1940/Q
genomic island; Leptospira interrogans;extrachromosomal circular plasmid
Lili Huang, Weinan Zhu, Ping He,Yan Zhang, Xuran Zhuang, Guoping Zhao, Xiaokui Guoa, Jinhong Qin, and Yongzhang Zhu. 1.Department of Microbiology and Immunology, Institute of Medical Science, Shanghai Jiao Tong University School of Medicine, 280 South Chongqing Road, Shanghai 200025, China 2CAS-Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China tThese authors contributed equally to this work. ＊Correspondence address. Tel： ＋86-21-63846590-778017; Fax： ＋86-21-64453285; E-mail： yzhzhu@hotmail.com （Y.Z.）/jinhongqin@sjtu.edu.cn （J.Q.）
In China, Leptospira interrogans serovar Lai strain 56601 （str.56601） is one of main pathogenic strains that cause severe leptospirosis in both human and animals. The genome of this organism was completely sequenced in 2003. However, in 2011, we identified and corrected some assembly errors in the str.56601 genome due to the repeat sequences widely distributed in the Leptospira genome. In this study, we re-analyzed the previously reported mobile, phage-related genomic island in the chromosome and rectified detailed sequence information in both the plasmid and chromosome using various experimental methods. The presence of a separate circular extrachromosomal plasmid was also confirmed, and its location in the genomi~ region was determined relative to the genomic island reported in L. interrogans serovar Lai by a combination of pulsed-field gel electrophoresis -based and plasmid extraction-based Southern blot analysis. This report confirmed that the sep arate extrachromosomal circular plasmid is not integrated into the chromosome of. interrogans str.56601 and markedly improved our understanding of the genomic organization, evolution, and pathogenesis of L. interrogans. In particular, characterization of this extrachromosomal cir cular plasmid will contribute to the development of genetic manipulation systems in pathogenic Leptospira species.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1672-9145
1745-7270
1745-7270
DOI:10.1093/abbs/gmu033