Biosynthesis of poly(2-hydroxybutyrate-co-lactate) in metabolically engineered Escherichia coli

• Published in: Biotechnology and bioprocess engineering Vol. 21; no. 1; pp. 169 - 174
• Main Authors: Chae, Cheol Gi, Kim, You Jin, Lee, Se Jin, Oh, Young Hoon, Yang, Jung Eun, Joo, Jeong Chan, Kang, Kyoung Hee, Jang, Young-Ah, Lee, Hyuk, Park, A-Reum, Song, Bong Keun, Lee, Sang Yup, Park, Si Jae
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.01.2016; Springer Nature B.V; 한국생물공학회
• Subjects: Biosynthesis; Biotechnology; Carbon; Carbon sources; Chemistry; Chemistry and Materials Science; Clostridium propionicum; E coli; Engineering; Environmental impact; Escherichia coli; Fermentation; Genes; Genetic engineering; Glucose; Industrial and Production Engineering; Metabolism; Mutation; NMR; Nuclear magnetic resonance; Plasmids; Polyhydroxyalkanoates; Polylactic acid; Polymers; Pseudomonas; Renewable resources; Research Paper; Sodium; Studies; 생물공학; South Korea; 2-hydroxybutyrate; PHA; recombinant; 2-hydroxyacid; lactate; poly(2-hydroxybutyrate-co-lactate)
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-015-0749-x

We have previously reported in vivo biosynthesis of polyhydroxyalkanoates containing 2-hydroxyacid monomers such as lactate and 2-hydroxybutyrate in recombinant Escherichia coli strains by the expression of evolved Clostridium propionicum propionyl-CoA transferase (PctCp) and Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate (PHA) synthase 1 (PhaC1 Ps 6-19 ). Here, we report the biosynthesis of poly(2-hydroxybutyrate-co-lactate)[P(2HB-co-LA)] by direct fermentation of metabolically engineered E. coli strain. Among E. coli strains WL3110, XL1-Blue, and BL21(DE3), recombinant E. coli XL1-Blue strain expressing PhaC1437 and Pct540 produced P(76.4mol%2HB-co-23.6mol%LA) to the highest content of 88 wt% when it was cultured in a chemically defined medium containing 20 g/L of glucose and 2 g/L of sodium 2-hydroxybutyrate. When recombinant E. coli XL1-Blue strain expressing PhaC1437 and Pct540 was cultured in a chemically defined medium containing 20 g/L of glucose and varying concentration of sodium 2-hydroxybutyrate, 2HB monomer fraction in P(2HB-co-LA) increased proportional to the concentration of sodium 2-hydroxybutyrate added to the culture medium. P(2HB-co-LA)] could also be produced from glucose as a sole carbon source without sodium 2-hydroxybutyrate into the culture medium. Recombinant E. coli XL1-Blue strain expressing the phaC1437 , pct540 , cimA3.7 , and leuBCD genes together with the L. lactis Il1403 panE gene, successfully produced P(23.5mol%2HB-co-76.5mol%LA)] to the polymer content of 19.4 wt% when it cultured in a chemically defined medium containing 20 g/L of glucose. The metabolic engineering strategy reported here should be useful for the production of novel copolymer P(2HB-co-LA)].