Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design

Aptamer-based fluorescence detection of platelet-derived growth factor (PDGF) on a functionalized diamond surface was demonstrated. In this work, a sandwich design based on the ability of PDGF to bind with aptamers at its two available binding sites was employed. It was found that this sandwich desi...

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Bibliographic Details
Published inAnalyst (London) Vol. 137; no. 7; pp. 1692 - 1697
Main Authors RAHIM RUSLINDA, A, PENMATSA, Varun, ISHII, Yoko, TAJIMA, Shinya, KAWARADA, Hiroshi
Format Journal Article
LanguageEnglish
Published Cambridge Royal Society of Chemistry 07.04.2012
Subjects
Affinity
Analytical chemistry
Aptamers, Nucleotide - chemistry
Binding
Binding sites
Biosensing Techniques - methods
Buffer solutions
Chemistry
Diamond - chemistry
Exact sciences and technology
Fluorescence
Limit of Detection
Platelet-derived growth factor
Platelet-Derived Growth Factor - analysis
Platelet-Derived Growth Factor - chemistry
Proteins - analysis
Proteins - chemistry
Reproducibility
Spectrometric and optical methods
Surface Properties
Fluorescence detector
Fluorescence
Diamond
Binding site
Selectivity
Concentration
Buffer solution
Fluorescence spectrometry
Design
Target
Signal
Platelet
Ionic strength
Reproducibility
Detection limit
Affinity
Detection
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Summary:Aptamer-based fluorescence detection of platelet-derived growth factor (PDGF) on a functionalized diamond surface was demonstrated. In this work, a sandwich design based on the ability of PDGF to bind with aptamers at its two available binding sites was employed. It was found that this sandwich design approach significantly increases the fluorescence signal intensity, and thereby a very low detection limit of 4 pM was achieved. The effect of the ionic strength of MgCl(2) buffer solution was also investigated, and the most favourable binding for PDGF-BB occurred at a Mg(2+) concentration of 5.5 mM. Since the aptamers bind to the target PDGF with high affinity, fluorescence detection exhibited high selectivity towards different biomolecules. The high reproducibility of detection was confirmed by performing three cycles of measurements over a period of three days.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0003-2654
1364-5528
DOI:10.1039/c2an15933c