The interactions of plant growth regulators and H2O2 during germination improvement of sweet corn seed through spermidine application

• Published in: Plant growth regulation Vol. 85; no. 1; pp. 15 - 26
• Main Authors: Huang, Yutao, Zhang, Yuchan, Gao, Canhong, Li, Zhan, Guan, Yajing, Hu, Weimin, Hu, Jin
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.05.2018; Springer Nature B.V
• Subjects: Abscisic acid; Acetylcysteine; Agriculture; Biomedical and Life Sciences; Biosynthesis; Calcium; Corn; Crosstalk; Germination; Growth regulators; Hydrogen peroxide; Inhibitors; Life Sciences; Metabolism; Original Paper; Plant Anatomy/Development; Plant growth; Plant Physiology; Plant Sciences; Seed germination; Soaking; Spermidine; Vegetables; Vigor; Seed vigor; H; Plant growth regulation; Polyamines; Crosstalk; O
• ISSN: 0167-6903; 1573-5087
• DOI: 10.1007/s10725-018-0370-z

Low seed vigor was the main constraint on the production of sweet corn in China. Spermidine (Spd) was proved to enhance sweet corn seed germination. However, little was known about the metabolisms and interactions of plant growth regulators (PGRs) and H 2 O 2 in the enhancement of Spd upon sweet corn seed germination. Spd, GA, C 2 H 4 and H 2 O 2 soaking treatments significantly enhanced seed vigor; while their respective biosynthesis inhibitors and ABA significantly declined seed vigor. Besides, as compared with control, seed vigor showed no significant difference in Spd+ProG (prohexadione-calcium, the inhibitor of GA), however it decreased significantly in Spd+ABA. The seed vigor treated by Spd+AVG (aviglycine hydrochloride, the inhibitor of C 2 H 4 ) and Spd+NAC ( n -acetyl-l-cysteine, a scavenger of H 2 O 2 ) were significantly lower than those soaked in Spd solution, but still significantly higher than the control. Spd+NAC with significantly lower H 2 O 2 content still up-regulated GA and C 2 H 4 contents and down-regulated ABA content during seed germination. The results suggested that it was Spd rather than H 2 O 2 (produced through Spd) made a direct effect on PGRs metabolism regulation in seed germination enhancement by Spd. The metabolism of GA and ABA played crucial rolesas compared with C 2 H 4 and H 2 O 2 . Besides, complicated PGRs interactions and crosstalk between H 2 O 2 and PGRs existed during sweet corn seed germination after Spd soaking, and ABA might be a key hormone in this process.