Endothelial NO/cGMP-dependent vascular relaxation of cornuside isolated from the fruit of Cornus officinalis

• Published in: Planta medica Vol. 73; no. 14; pp. 1436 - 1440
• Main Authors: Kang, D.G, Choi, D.H, Lee J.K, Lee, Y.J, Moon, M.K, Yang, S.N, Kwon, T.O, Kwon, J.W, Kim, J.S, Lee, H.S
• Format: Journal Article
• Language: English
• Published: Germany 01.11.2007
• Subjects: Animals; aorta; Aorta - drug effects; Cornaceae; Cornus - chemistry; Cornus officinalis; cornuside; cultured cells; cyclic GMP; Cyclic GMP - metabolism; Dose-Response Relationship, Drug; endothelial cells; endothelium; Endothelium, Vascular - drug effects; Endothelium, Vascular - physiology; Fruit - chemistry; glucosides; Glucosides - chemistry; Glucosides - isolation & purification; Glucosides - pharmacology; humans; Male; Molecular Structure; nitric oxide; Nitric Oxide - metabolism; pyrans; Pyrans - chemistry; Pyrans - isolation & purification; Pyrans - pharmacology; Rats; Rats, Sprague-Dawley; vasodilation; Vasodilation - drug effects; South Korea
• ISSN: 0032-0943; 1439-0221
• DOI: 10.1055/s-2007-990243

Cornuside is a bisiridoid glucoside compound isolated from the fruit of CORNUS OFFICINALIS Sieb. et Zucc. (Cornaceae). In the present study, we investigated the effect of cornuside on vascular tone in rat aortic tissue. Cornuside induced a concentration-dependent relaxation of the phenylephrine-precontracted rat aorta, which was abolished by removal of the endothelial layer. Pretreatment of the aortic tissues with either N(G)-nitro- L-arginine methyl ester (L-NAME) or 1 H- -oxadiazole-[4,3-alpha]-quinoxalin-1-one (ODQ) completely inhibited the relaxation induced by cornuside. However, the relaxant effect of cornuside was not blocked by pretreatment with verapamil, diltiazem, tetraethylammonium (TEA), glibenclamide, indomethacin, atropine, or propranolol. In addition, incubation of human umbilical vein endothelial cells (HUVECs) with cornuside increased the production of cGMP in a dose-dependent manner, but this effect was blocked by pretreatment with L-NAME and ODQ, respectively. Taken together, the present study suggests that cornuside dilates vascular smooth muscle via endothelium-dependent nitric oxide (NO)/cGMP signaling.