Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout (Oncorhynchus Mykiss) and Brown Trout (Salmo Trutta Morpha fario)

• Published in: Journal of visualized experiments no. 139
• Main Authors: Kowalski, Radosław Kajetan, Sarosiek, Beata, Judycka, Sylwia, Dryl, Katarzyna, Grudniewska, Joanna, Dobosz, Stefan, Cejko, Beata Irena
• Format: Journal Article
• Language: English
• Published: United States MyJove Corporation 16.09.2018
• Subjects: Air; Animals; Developmental Biology; Oncorhynchus mykiss; Ovum; Reproducibility of Results; Specimen Handling - methods
• ISSN: 1940-087X; 1940-087X
• DOI: 10.3791/56894

Egg collection is one of the most crucial procedures during fish reproduction in salmonid hatcheries. Classic methods involve the use of hand massage on fish abdomens to expel the eggs. An alternative method uses the pressure of gas injected into the body cavity, which causes the subsequent release of the eggs. This method is believed to have less negative effects on both the welfare and egg quality of the broodstocks. Herein, we compare the results of air and hand stripping methods with respect to one-year survival and egg quantity and quality in two salmonid fish, rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta morpha fario). Our results indicate that air stripping yielded a better quality of eggs and higher one-year survival rate in rainbow trout. In addition, air stripping resulted in lower mortality rate than the group subjected to hand stripping (25% vs. 35%). The pH and hatching rate of the hand stripped group was lower than those of the air stripped group. In the case of brown trout, the quality of eggs obtained by both hand and air-stripping methods was similar; however, the one-year losses in fish were higher in air stripped group (15% compared to 0% in hand stripped fish). Although the advantages of air stripping method over hand stripping in terms of egg quality might not be observed in all salmonid species, the air-stripping procedure might be a promising option to be adopted in hatcheries as it ensures a high level of reproducibility and efficiency.