DNA Copy Number Alterations and Copy Neutral Loss of Heterozygosity in Adult Ph-Negative Acute B-Lymphoblastic Leukemia: Focus on the Genes Involved

• Published in: International journal of molecular sciences Vol. 24; no. 24; p. 17602
• Main Authors: Risinskaya, Natalya, Gladysheva, Maria, Abdulpatakhov, Abdulpatakh, Chabaeva, Yulia, Surimova, Valeriya, Aleshina, Olga, Yushkova, Anna, Dubova, Olga, Kapranov, Nikolay, Galtseva, Irina, Kulikov, Sergey, Obukhova, Tatiana, Sudarikov, Andrey, Parovichnikova, Elena
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.12.2023
• Subjects: acute B-lymphoblastic leukemia (B-ALL); Cell cycle; Chromosomes; Cloning; copy neutral loss of heterozygosity (cnLOH); copy number alterations (CNAs); Genes; Genomes; Leukemia; molecular karyotype; Patients; copy neutral loss of heterozygosity (cnLOH); acute B-lymphoblastic leukemia (B-ALL); molecular karyotype; genes; copy number alterations (CNAs)
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms242417602

The landscape of chromosomal aberrations in the tumor cells of the patients with B-ALL is diverse and can influence the outcome of the disease. Molecular karyotyping at the onset of the disease using chromosomal microarray (CMA) is advisable to identify additional molecular factors associated with the prognosis of the disease. Molecular karyotyping data for 36 patients with Ph-negative B-ALL who received therapy according to the ALL-2016 protocol are presented. We analyzed copy number alterations and their prognostic significance for / , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , and genes, choosing t(4;11) and t(7;14) as reference events. Of the 36 patients, only 5 (13.8%) had a normal molecular karyotype, and 31 (86.2%) were found to have various molecular karyotype abnormalities-104 deletions, 90 duplications or amplifications, 29 cases of cnLOH and 7 biallelic/homozygous deletions. We found that 11q22-23 duplication involving the , and genes was the most adverse prognostic event in the study cohort.