Oxidation of low-density lipoproteins: intraindividual variability and the effect of dietary linoleate supplementation
Low-density lipoprotein (LDL) oxidation was measured in vitro to determine intraindividual variability and to relate oxidation to linoleic acid enrichment. Intraindividual variability was determined for eight subjects on 3 consecutive days after 14 d on a fixed diet. Coefficients of variation were 7...
Saved in:
Published in | The American journal of clinical nutrition Vol. 57; no. 3; pp. 391 - 398 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.03.1993
American Society for Clinical Nutrition, Inc |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Low-density lipoprotein (LDL) oxidation was measured in vitro to determine intraindividual variability and to relate oxidation to linoleic acid enrichment. Intraindividual variability was determined for eight subjects on 3 consecutive days after 14 d on a fixed diet. Coefficients of variation were 7.49 ± 1.50%, 6.58 ± 1.16%, and 4.58 ± 0.77% for oxidation rate, lag time, and diene concentration, respectively. In the second study 12 normolipidemic men consumed a daily diet supplement containing 35 g linoleate-rich oil in one period and 35 g oleate-rich oil in the other period (2 × 3 wk crossover). LDL oxidized faster after the linoleate diet than after the oleate diet (x̄ ± SE: 16.42 ± 0.85 and 13.16 ± 0.68 nmol diene · mg LDL protein−1 · min−1, respectively, P < 0.02) and produced more conjugated diene (416 ± 12.60 and 379.29 ± 11.06 nmol/mg protein, respectively, P < 0.05) in proportion to the increase in LDL linoleate (r = 0.698, P < 0.001 and r = 0.618, P < 0.01 for rate and diene concentration, respectively). Lag time before onset of oxidation was not significantly altered by the linoleate-rich diet. |
---|---|
Bibliography: | S20 9404829 S30 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0002-9165 1938-3207 |
DOI: | 10.1093/ajcn/57.3.391 |