Oxidation of low-density lipoproteins: intraindividual variability and the effect of dietary linoleate supplementation

• Published in: The American journal of clinical nutrition Vol. 57; no. 3; pp. 391 - 398
• Main Authors: Abbey, M, Belling, GB, Noakes, M, Hirata, F, Nestel, PJ
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.1993; American Society for Clinical Nutrition, Inc
• Subjects: ACIDE LINOLEIQUE; ACIDO LINOLEICO; ADDITIF ALIMENTAIRE; ADITIVOS ALIMENTARIOS; antioxidants; beta Carotene; Carotenoids - administration & dosage; CHOLESTEROL; COLESTEROL; conjugated dienes; Copper - pharmacology; copper oxidation; Copper Sulfate; Diet; Dietary Fats, Unsaturated - administration & dosage; Dietary Fats, Unsaturated - pharmacology; Energy Intake; Fatty Acids - blood; Fatty Acids, Monounsaturated - administration & dosage; Fatty Acids, Monounsaturated - pharmacology; Fatty Acids, Unsaturated - administration & dosage; Fatty Acids, Unsaturated - pharmacology; Female; FEMME; HOMBRES; HOMME; Humans; Kinetics; Linoleic Acid; Linoleic Acids - administration & dosage; Linoleic Acids - pharmacology; LIPEMIA; LIPEMIE; LIPOPROTEINAS; LIPOPROTEINE; Lipoproteins, LDL - blood; Male; Medical research; monounsaturated fatty acids; MUJERES; Oleic Acid; Oleic Acids - administration & dosage; Oleic Acids - pharmacology; OXIDACION; Oxidation; Oxidation-Reduction; Oxidized low-density lipoprotein; OXYDATION; plasma lipids; PLASMA SANGUIN; PLASMA SANGUINEO; polyunsaturated fatty acids; Proteins; TRIGLICERIDOS; TRIGLYCERIDE; Vitamin E - administration & dosage; conjugated dienes; Oxidized low-density lipoprotein; antioxidants; copper oxidation; polyunsaturated fatty acids; monounsaturated fatty acids; plasma lipids
• ISSN: 0002-9165; 1938-3207
• DOI: 10.1093/ajcn/57.3.391

Low-density lipoprotein (LDL) oxidation was measured in vitro to determine intraindividual variability and to relate oxidation to linoleic acid enrichment. Intraindividual variability was determined for eight subjects on 3 consecutive days after 14 d on a fixed diet. Coefficients of variation were 7.49 ± 1.50%, 6.58 ± 1.16%, and 4.58 ± 0.77% for oxidation rate, lag time, and diene concentration, respectively. In the second study 12 normolipidemic men consumed a daily diet supplement containing 35 g linoleate-rich oil in one period and 35 g oleate-rich oil in the other period (2 × 3 wk crossover). LDL oxidized faster after the linoleate diet than after the oleate diet (x̄ ± SE: 16.42 ± 0.85 and 13.16 ± 0.68 nmol diene · mg LDL protein−1 · min−1, respectively, P < 0.02) and produced more conjugated diene (416 ± 12.60 and 379.29 ± 11.06 nmol/mg protein, respectively, P < 0.05) in proportion to the increase in LDL linoleate (r = 0.698, P < 0.001 and r = 0.618, P < 0.01 for rate and diene concentration, respectively). Lag time before onset of oxidation was not significantly altered by the linoleate-rich diet.