Terbufos sulfone aggravates kidney damage in STZ-induced diabetic rats

• Published in: Biológia Vol. 72; no. 8; pp. 946 - 953
• Main Authors: Nurulain, Syed Muhammad, Ojha, Shreesh, Shafiullah, Mohamed, Yasin, Javed, Yasmin, Tayyaba, Saeed, Tariq, Adeghate, Ernest
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.08.2017; De Gruyter; Springer Nature B.V
• Subjects: Acetylcholinesterase; animal disease models; Biochemistry; Biocompatibility; Blood; blood glucose; blood serum; Body weight; Cell Biology; Chronic exposure; Diabetes; Diabetes mellitus; enzyme inhibition; Erythrocytes; Glucose; glycemic effect; Insecticides; Kidneys; Lethal dose; lethal dose 50; Life Sciences; males; Microbiology; nephrotoxicity; Organophosphates; Organophosphorus compounds; Plant Sciences; poisoning; Rats; red-blood-cell-acetylcholinesterase; Rodents; Streptozocin; streptozotocin; Structural damage; terbufos; terbufos-sulfone; Toxicity; Transmission electron microscopy; urinalysis; Urine; Zoology; transmission electron microscopy; nephrotoxicity; diabetes; terbufos-sulfone; red-blood-cell-acetylcholinesterase
• ISSN: 0006-3088; 1336-9563; 1336-9563
• DOI: 10.1515/biolog-2017-0106

The consequences of chronic exposure of organophosphorus compounds (OPCs) on diabetic subjects have been seldom reported. The aim of the present study was to assess the impact of non-lethal dose of terbufos sulfone (TS), an organophosphate, on the kidney of non-diabetic and streptozotocin (STZ)- induced diabetic rats. The diabetogenic effect of TS was also examined. Male Wistar rats were treated for two weeks with 130 µg/kg body weight/day of TS. This dose was 1/20 of LD 50 and produces less than 10% inhibition of acetylcholinesterase (AChE) in non-diabetic rats. No observable symptoms of poisoning were noted. Weight, glucose and in vivo red blood cell (RBC)-AChE were measured on day 7 and 15 of the study. Serum biochemistry, urine analysis, and transmission electron microscopy (TEM) of kidney were performed to assess the toxicity at the end of the 15-day study. Our results did not reveal diabetogenic effect of TS treatment. Blood glucose level was significantly increased and RBC-AChE activity was significantly decreased in diabetic-TS treated rats compared to untreated diabetic and normal control animals. TEM of kidney revealed structural damage to kidney, which was more severe in diabetic-TS treated rats compared to controls. Toxicity to kidney by TS was further confirmed by urine and blood biochemistry. Our results conclude that non-lethal dose of TS aggravates the nephrotoxicity in diabetic rats. Further studies with other OPCs are needed to be able to generalize the diabetogenic effect of TS.