Sulphur deficiency inhibits nitrogen assimilation and recycling in barley plants

• Published in: Biologia plantarum Vol. 61; no. 4; pp. 675 - 684
• Main Authors: Veliz, C. G., Roberts, I. N., Criado, M. V., Caputo, C.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.12.2017; Springer Nature B.V; Institute of Experimental Botany of the Czech Academy of Sciences
• Subjects: amino acid transporters; Amino acids; Assimilation; Availability; Barley; Biological assimilation; Biomedical and Life Sciences; Cereals; Correlation analysis; Exudation; Gene expression; Gene regulation; Genes; Glutamate-ammonia ligase; Glutamine; glutamine synthetase; hordeum vulgare; Life Sciences; Metabolites; Nitrogen; Nutrients; Original Paper; Phloem; phloem amino acid exudation; Plant Sciences; Plants (botany); Recycling; Secondary metabolites; Soil nutrients; Sulfur; Tissues; glutamine synthetase; phloem amino acid exudation; amino acid transporters
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/s10535-017-0722-y

Sulphur (S) is incorporated into diverse primary and secondary metabolites that play important roles in proper growth and development of plants. In cereals, a fraction of the nitrogen (N) accumulated in developing grains is guaranteed by amino acid remobilization from vegetative tissues, a contribution that becomes critical when soil nutrients are deficient. Glutamine synthetase (GS) and amino acid transporters (AAT) are key components involved in N assimilation and recycling. The aim of the present study was to evaluate the effect of S availability on the expressions of HvGS and several selected HvAAT genes in barley plants and on the phloem exudation rate of amino acids. To this end, two independent experiments were designed to impose low S availability conditions to barley plants. Low S availability caused a decrease in the phloem exudation rate of amino acids as well as in the gene expression of all the HvGS genes and five of the six HvAAT genes analyzed. The strong correlation found between the phloem amino acid exudation rate and HvGS1-1 , HvGS1-2 , HvAAP7 , and HvProT1 gene expression may indicate the participation of these genes in the regulation of amino acid remobilization through the phloem.