Short term performance and microbial community of a sulfide-based denitrification and Anammox coupling system at different N/S ratios

• Published in: Bioresource technology Vol. 294; p. 122130
• Main Authors: Qin, Yujie, Wu, Chenglong, Chen, Buqing, Ren, Junyi, Chen, Linyi
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.12.2019
• Subjects: Anammox; Microbial community; Nitrogen removal; Sulfide removal; Sulfide-dependent autotrophic denitrification; Nitrogen removal; Anammox; Sulfide removal; Sulfide-dependent autotrophic denitrification; Microbial community
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2019.122130

[Display omitted] •A novel SDAD-Anammox process was developed for nitrogen and sulfide removal.•Short term effects of different N/S ratios on SDAD-Anammox activity was obvious.•The best TN removal efficiency was 82.8% at a N/S ratio of 2.38.•Denitrification contributed to N loss improved with the N/S ratios decreased.•Candidatus Kuenenia and Thiobacillius became dominated when nitrate was limited. A novel sulfide-based denitrification and Anammox process was established for simultaneous removal of nitrogen and sulfide in a UBF reactor. The effects of the N/S ratio on reactor performance were investigated under five N/S molar ratios (4.56, 2.38, 0.96, 0.73, and 0.51). The best total nitrogen removal efficiency was 82.8% at a N/S ratio of 2.38. When the N/S ratio exceeded 0.96, Anammox contributed to more than 90% of the N loss. Sulfide was completely removed during the full operational period and S0 accumulation occurred when N/S ratio was less than 1. Thiobacillus (6.1%) and Candidatus Kuenenia (18.8%) were the main functional microorganisms when nitrate was in excess on day 12. As nitrate became limited on day 50, Thiobacillus (21.0%), Sulfurimonas (3.9%), and Candidatus Kuenenia (19.7%) became dominated. In this study, Candidatus Kuenenia was not inhibited by the sulfide.