Kordiimonas aestuarii sp. nov., a marine bacterium isolated from a tidal flat

• Published in: International journal of systematic and evolutionary microbiology Vol. 62; no. Pt 12; pp. 3049 - 3054
• Main Authors: Math, Renukaradhya K, Jeong, Sang Hyeon, Jin, Hyun Mi, Park, Moon Su, Kim, Jeong Myeong, Jeon, Che Ok
• Format: Journal Article
• Language: English
• Published: Reading International Union of Microbiological Societies 01.12.2012; Society for General Microbiology
• Subjects: Alphaproteobacteria - classification; Alphaproteobacteria - genetics; Alphaproteobacteria - isolation & purification; Animal, plant and microbial ecology; Bacteria; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; DNA; DNA, Bacterial - genetics; fatty acids; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; genes; Geologic Sediments - microbiology; Microbial ecology; Microbiology; Molecular Sequence Data; new species; Nucleic Acid Hybridization; nucleotide sequences; Phylogeny; Republic of Korea; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Sequence Analysis, DNA; sodium chloride; Systematics; Ubiquinone - analysis; Various environments (extraatmospheric space, air, water); Korean Peninsula; Republic of Korea; INW, Korea, Rep; Marine environment; Bacteria; New species; Tidal flat; Taxonomy
• ISSN: 1466-5034; 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.038943-0

A Gram-staining negative, strictly aerobic bacterium, designated 101-1T, was isolated from a sea tidal flat, Taean, Korea. The strain formed small light-yellow, smooth, and circular colonies on marine agar. Cells were weakly halophilic, motile rods showing catalase- and oxidase-positive reactions. Growth of strain 101-1T was observed at 15–40 °C (optimum, 30 °C), pH 5.0–8.0 (optimum, pH 6.5–7.0) and 1.0–9.0 % (w/v) NaCl (optimum, 2.0–3.5 %). The G+C content of the genomic DNA was 53.3 mol%. Strain 101-1T contained ubiquinone-10 (Q-10) as the respiratory quinone and iso-C17 : 1ω9c, iso-C15 : 0 and iso-C17 : 0 as major fatty acids. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 101-1T formed a tight phylogenetic lineage with members of the genus Kordiimonas and was most closely related to Kordiimonas gwangyangensis GW14-5T and Kordiimonas lacus S3-22T with 97.3 % and 96.3 % 16S rRNA gene sequence similarities, respectively. The DNA–DNA relatedness values between strain 101-1T and K. gwangyangensis GW14-5T and K. lacus S3-22T were 24.8±4.4 % and 32.2±3.6 %, respectively. Based on the data from the phenotypic and genotypic studies, strain 101-1T represents a novel species of the genus Kordiimonas, for which the name Kordiimonas aestuarii sp. nov. is proposed. The type strain is 101-1T ( = KACC 16184T = JCM 17742T).