Amino acid transport in protoplasts isolated from soybean leaves

• Published in: Plant physiology (Bethesda) Vol. 81; no. 1; pp. 8 - 11
• Main Authors: VerNooy, C.D, Lin, W
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.05.1986
• Subjects: ABSORCION DE SUBSTANCIAS NUTRITIVAS; ABSORPTION DE SUBSTANCES NUTRITIVES; Absorption. Translocation of ions and substances. Permeability; ACIDE AMINE; Agronomy. Soil science and plant productions; Amino acid metabolism; AMINO ACIDS; AMINOACIDOS; Biological and medical sciences; Economic plant physiology; FEUILLE; Fundamental and applied biological sciences. Psychology; GLYCINE MAX; HOJAS; Kinetics; LEAVES; MESOFILO; MESOPHYLL; Mesophyll cells; MESOPHYLLE; Metabolism; Metabolism. Physicochemical requirements; NUTRIENT UPTAKE; Nutrition. Photosynthesis. Respiration. Metabolism; Plant physiology and development; Plants; PROTOPLASM; PROTOPLASMA; PROTOPLASME; Protoplasts; Soybeans; Translocation; Leguminosae; Dicotyledones; Aminoacid; Protoplast; Angiospermae; Spermatophyta; Glycine max; Oil plant(vegetal)
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.81.1.8

We isolated large quantities of mesophyll protoplasts from source and sink leaves of soybean plants and examined them for amino acid uptake. Accumulation of amino acids in isolated protoplasts was linear for at least 40 minutes. Uptake kinetics revealed the presence of both saturable and linear components. Increasing external pH decreases the uptake. The uncoupler, carbonyl cyanide p-trifluoromethoxyphenylhydrazone at 15 micromolar inhibited and fusicoccin at 10 micromolar stimulated amino acid uptake. Our data are consistent with a proton-cotransport mechanism for the uptake of L-glutamine and α-amino isobutyric acid into soybean mesophyll cells.