A Highly Sensitive, Excimer-Controlled Molecular Beacon

Non‐nucleosidic chromophores in the stem of a molecular beacon inhibit excimer fluorescence through the formation of a donor–acceptor complex (see picture). The excellent reduction of the background fluorescence allows the detection of DNA and RNA targets in the presence of a significant excess of t...

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Bibliographic Details
Published inAngewandte Chemie (International ed.) Vol. 49; no. 7; pp. 1227 - 1230
Main Authors Häner, Robert, Biner, Sarah M., Langenegger, Simon M., Meng, Tao, Malinovskii, Vladimir L.
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 08.02.2010
WILEY‐VCH Verlag
Subjects
Base Sequence
DNA
DNA - analysis
donor-acceptor systems
fluorescence
Fluorescent Dyes - chemistry
Imides - chemistry
molecular beacons
Nucleic Acid Hybridization
Oligonucleotide Probes - chemistry
Perylene - analogs & derivatives
Perylene - chemistry
Pyrenes - chemistry
RNA
RNA - analysis
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Summary:Non‐nucleosidic chromophores in the stem of a molecular beacon inhibit excimer fluorescence through the formation of a donor–acceptor complex (see picture). The excellent reduction of the background fluorescence allows the detection of DNA and RNA targets in the presence of a significant excess of the probe.
Bibliography:istex:241A95120DC5BABE3EA8CF0C9FEE0C6D3DEA4A3D
ArticleID:ANIE200905829
This work was supported financially by the Swiss National Foundation (grant 200020-117617).
ark:/67375/WNG-T3RXG0RS-1
Swiss National Foundation - No. 200020-117617
This work was supported financially by the Swiss National Foundation (grant 200020‐117617).
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200905829