Preparative affinity membrane electrophoresis

Using the patented Gradiflow system in conjunction with newly developed affinity membranes, the suitability of an electrokinetic technique for affinity fractionation was investigated. Blue dextran incorporated into an affinity membrane was used to deplete a solution of horse serum of albumin, with t...

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Bibliographic Details
Published inElectrophoresis Vol. 17; no. 1; p. 224
Main Authors Horvath, Z S, Gooley, A A, Wrigley, C W, Margolis, J, Williams, K L
Format Journal Article
LanguageEnglish
Published Germany 01.01.1996
Subjects
Blood Proteins - analysis
Chromatography, Affinity
Coloring Agents
Dextrans
Electrochemistry
Electrophoresis, Polyacrylamide Gel - methods
Kinetics
Membranes, Artificial
Serum Albumin - analysis
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Summary:Using the patented Gradiflow system in conjunction with newly developed affinity membranes, the suitability of an electrokinetic technique for affinity fractionation was investigated. Blue dextran incorporated into an affinity membrane was used to deplete a solution of horse serum of albumin, with the result that a majority of serum proteins were enriched tenfold relative to albumin. The technique, when fully developed, would offer some advantages over affinity chromatography, since to a degree it is possible to control which components of the sample are presented to the affinity matrix. Furthermore, the technique would extend the capabilities of the already multifunctional Gradiflow system.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.1150170138