Rapid detection of t(15;17)(q24;q21) in acute promyelocytic leukaemia by microwave‐assisted fluorescence in situ hybridization

• Published in: Hematological oncology Vol. 35; no. 1; pp. 94 - 100
• Main Authors: Soriani, Silvia, Mura, Cinzia, Panico, Anna Rita, Scarpa, Anna Maria, Recchimuzzo, Patrizia, Dadati, Raffaella, Farioli, Renata, De Canal, Gabriella, Mura, Maria Angela, Cesana, Clara
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.03.2017
• Subjects: acute promyelocytic leukaemia; Chromosomes, Human, Pair 15; Chromosomes, Human, Pair 17; DNA Probes; DNA, Neoplasm - analysis; Female; fluorescence in situ hybridization; Gene Rearrangement; Humans; Immunophenotyping; In Situ Hybridization; In Situ Hybridization, Fluorescence; Karyotyping; Leukemia, Promyelocytic, Acute - genetics; Male; microwave oven; Microwaves; Oncogene Proteins, Fusion - genetics; Translocation, Genetic; fluorescence in situ hybridization; acute promyelocytic leukaemia; microwave oven
• ISSN: 0278-0232; 1099-1069
• DOI: 10.1002/hon.2194

Acute promyelocytic leukaemia (APL) is a hematologic malignancy characterized by the rearrangement of the PML and RARα genes, mostly due to a reciprocal chromosomal translocation t(15;17)(q24;q21). A quick APL diagnosis is essential for starting a prompt suitable therapy. We describe a new rapid diagnostic laboratory approach to detect the PML‐RARα rearrangement, which gives clear genetic results within 30 min of hybridization. It combines quick cell harvesting, fluorescence in situ hybridization performed with commercial DNA probe and microwave beams supplied by a domestic microwave oven. Copyright © 2015 John Wiley & Sons, Ltd.