Generation and selection of monoclonal antibodies identifying surface molecules discriminating between fetal and adult fibroblasts

• Published in: Journal of clinical laboratory analysis Vol. 3; no. 1; pp. 50 - 55
• Main Authors: Funaro, A., Bellone, G., Demonte, L., Alessio, M., Roggero, S., Peruui, L., Cappa, A. P. M., Malavasi, F.
• Format: Journal Article
• Language: English
• Published: New York Wiley Subscription Services, Inc., A Wiley Company 1989
• Subjects: Adult; Amniocentesis; Antibodies, Monoclonal; Antigens, Surface; Cell Differentiation; Cell Line; feta/embryonic antigens; Fetus - immunology; Fibroblasts - cytology; Fibroblasts - immunology; Humans; monoclonal antibody; Prenatal Diagnosis; prenatal diagnostics; Tumor Cells, Cultured - immunology
• ISSN: 0887-8013; 1098-2825
• DOI: 10.1002/jcla.1860030110

The present report describes the reactivity of two murine monoclonal antibodies, i.e., CB11 and CB43, the first obtained by using embryonic material as immunizer and the second elicited against a human cell line. These reagents recognize two distinct molecules marking a peculiar differentiation stage on fetallembryonic fibroblasts. The first structure is a single chain of 14.5 kd, the second one a heterodimer of 40‐80 kd. Even if these reagents are not strictly fibroblast restricted, translineage expression does not diminish the medical relevance of the reagents; in fact, they may have application in prenatal diagnostics and amniocentesis testing, in which it is necessary to discriminate between fetal and maternal cells.