Mechanical stimulation induced osteogenic differentiation of BMSCs through TWIST/E2A/p21 axis

• Published in: Bioscience reports Vol. 40; no. 5
• Main Authors: Guo, Qingyuan, Liu, Ying, Sun, Renhao, Yang, Fang, Qiao, Pengyan, Zhang, Rong, Song, Ling, E, Lingling, Liu, Hongchen
• Format: Journal Article
• Language: English
• Published: England Portland Press Ltd 29.05.2020
• Subjects: Animals; Basic Helix-Loop-Helix Transcription Factors - genetics; Basic Helix-Loop-Helix Transcription Factors - metabolism; Bone Marrow Cells - metabolism; Cell Differentiation; Cells, Cultured; Cyclin-Dependent Kinase Inhibitor p21 - genetics; Cyclin-Dependent Kinase Inhibitor p21 - metabolism; Developmental Biology; Gene Expression Regulation; Male; Mechanotransduction, Cellular; Mesenchymal Stem Cells - metabolism; Osteogenesis; Rats, Sprague-Dawley; Stem Cells; Stress, Mechanical; Twist-Related Protein 1 - genetics; Twist-Related Protein 1 - metabolism; mechanical stimulation; osteogenic differentiation; BMSCs; p21
• ISSN: 0144-8463; 1573-4935
• DOI: 10.1042/BSR20193876

The relationship between mechanical force and alveolar bone remodeling is an important issue in orthodontics because tooth movement is dependent on the response of bone tissue to the mechanical force induced by the appliances used. Mechanical cyclical stretch plays an essential role in the cell osteogenic differentiation involved in bone remodeling. However, the underlying mechanisms are unclear, particularly the molecular pathways regulated by mechanical stimulation. In the present study, we reported a dynamic change of p21 level in response to mechanical cyclical stretch, and shRNA-p21 in bone marrow mesenchymal stem cells (BMSCs) induced osteogenic differentiation. The mechanism was mediated through TWIST/E2A/p21 axis. These results supported the mechanical stimulation-induced osteogenic differentiation is negatively regulated by p21.