The anti-inflammatory effects of 15-HETE on osteoarthritis during treadmill exercise

• Published in: Life sciences (1973) Vol. 273; p. 119260
• Main Authors: Tian, Yicheng, Gou, Jian, Zhang, He, Lu, Jinghan, Jin, Zhuangzhuang, Jia, Shuangshuo, Bai, Lunhao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15.05.2021; Elsevier BV
• Subjects: 1-Phosphatidylinositol 3-kinase; 15-HETE; ADAM protein; AKT protein; Animals; Anti-Inflammatory Agents - pharmacology; Arthritis; Arthritis, Experimental - chemically induced; Arthritis, Experimental - pathology; Arthritis, Experimental - prevention & control; Biomedical materials; Cartilage; Cartilage diseases; Chondrocyte; Chondrocytes; Collagen; Collagenase 3; Enzyme-linked immunosorbent assay; Fitness equipment; Hydroxyeicosatetraenoic Acids - pharmacology; IL-1β; Immunohistochemistry; Inflammation; Inflammation - chemically induced; Inflammation - drug therapy; Inflammation - pathology; Interleukins; Iodoacetic Acid - toxicity; Joint diseases; Joints (anatomy); Knee; Male; Matrix metalloproteinase; Matrix metalloproteinases; NF-kappa B - metabolism; Osteoarthritis; Osteoarthritis - chemically induced; Osteoarthritis - pathology; Osteoarthritis - prevention & control; Physical Conditioning, Animal; PI3k-Akt signalling; Polymerase chain reaction; Rats; Rats, Sprague-Dawley; Thrombospondin; Treadmill exercise; Treadmills; Western blotting; Treadmill exercise; 15-HETE; PI3k-Akt signalling; Chondrocyte; Osteoarthritis
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2021.119260

Investigate the involvement of 15-hydroxyeicosatetraenoic acid (15-HETE), an anti-inflammatory molecule, on the beneficial effects of exercise therapy for osteoarthritis (OA). 15-HETE (10 μM, twice a week) and monosodium iodoacetate (MIA) (1 mg) were injected into rat knee joints. Treadmill exercise was applied on OA rat. Primary rat chondrocytes were treated with 15-HETE, LY294002 and interleukin (IL)-1β. Rats undergo a 1 hour single session treadmill exercise once. 15-HETE levels in the knee joint were evaluated using ELISA after a single session of treadmill exercise on healthy and OA rats. Matrix metalloproteinase (MMP)3, MMP-13, a disintegrin and metalloproteinase with thrombospondin motif (ADAMTS)-5, p-Akt, Akt, and collagen type 2 (COL2) expression were evaluated using RT-PCR and western blotting. OA degree was evaluated using X-ray, scored by Osteoarthritis Research Society International (OARSI) and Mankin scores. COL2 and MMP-13 expression in articular was evaluated using immunohistochemistry. Medium intensity exercise alleviated OA. 15-HETE levels after exercise was increased. 15-HETE inhibited IL-1β-induced inflammation in primary chondrocytes and increased p-Akt levels. LY294002 blocked the effect of 15-HETE in vitro. Finally, 15-HETE alleviated cartilage damage, inhibited MMP-13 expression, and increased COL2 expression in joint cartilage tissue. Treadmill exercise alleviates OA and increases 15-HETE levels in the knee joint, which suppresses inflammation in chondrocytes via PI3k-Akt signalling in vitro and in vivo.