MiR-30e-5p inhibits the migration and invasion of nasopharyngeal carcinoma via regulating the expression of MTA1

• Published in: Bioscience reports Vol. 40; no. 5
• Main Authors: Hu, Weiqun, Yao, Wenfeng, Li, Haolin, Chen, Li
• Format: Journal Article
• Language: English
• Published: England Portland Press Ltd 29.05.2020
• Subjects: Adult; Cancer; Cell Line, Tumor; Cell Migration, Adhesion & Morphology; Cell Movement; Female; Gene Expression Regulation, Neoplastic; Humans; Male; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 3 - metabolism; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; Nasopharyngeal Carcinoma - genetics; Nasopharyngeal Carcinoma - metabolism; Nasopharyngeal Carcinoma - mortality; Nasopharyngeal Carcinoma - secondary; Nasopharyngeal Neoplasms - genetics; Nasopharyngeal Neoplasms - metabolism; Nasopharyngeal Neoplasms - mortality; Nasopharyngeal Neoplasms - pathology; Neoplasm Invasiveness; Repressor Proteins - genetics; Repressor Proteins - metabolism; RNA; Signal Transduction; Snail Family Transcription Factors - metabolism; Trans-Activators - genetics; Trans-Activators - metabolism; Vimentin - metabolism; Nasopharyngeal carcinoma; MTA1; MiR-30e-5p; Migration; Invasion
• ISSN: 0144-8463; 1573-4935
• DOI: 10.1042/BSR20194309

The study explored the effect of miR-30e-5p on nasopharyngeal carcinoma (NPC). MiR-30e-5p levels in NPC cancer and adjacent normal samples, in metastatic and non-metastatic cancer samples of NPC, and in NP69 cell and five NPC cell lines were determined by quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between miR-30e-5p and MTA1 was confirmed by dual-luciferase reporter assay, Western blot and qRT-PCR. The viability, migration and invasion of 5-8F and 6-10B cells were determined by CCK-8, scratch test and transwell assays, respectively. The levels of migration-related proteins (vimentin and Snail) and invasion-related proteins (MMP2 and MMP3) in NPC cells were detected by Western blot. The results showed that low expression of miR-30e-5p was associated with HNSC cancer, NPC, metastasis of NPC and NPC cell lines. Overexpressed miR-30e-5p in HNSC cancer and NPC was predictive of a better prognosis of patients. In addition, the viability, migration and invasion were reduced by up-regulating miR-30e-5p in 5-8F cells, but promoted by down-regulated miR-30e-5p in 6-10B cells. MiR-30e-5p reversed the migration and invasion of NPC cells regulated by MTA1, and inhibited migration and invasion of NPC cells via regulating MTA1 expression.