A GoldenBraid cloning system for synthetic biology in social amoebae

GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present Gol...

Full description

Saved in:
Bibliographic Details
Published inNucleic acids research Vol. 48; no. 8; pp. 4139 - 4146
Main Authors Kundert, Peter, Sarrion-Perdigones, Alejandro, Gonzalez, Yezabel, Katoh-Kurasawa, Mariko, Hirose, Shigenori, Lehmann, Peter, Venken, Koen J T, Shaulsky, Gad
Format Journal Article
LanguageEnglish
Published England Oxford University Press 07.05.2020
Subjects
Data Resources and Analyses
Online AccessGet full text

Cover

More Information
Summary:GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Passed away on 19 November 2019.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkaa185