An electrochemical impedimetric immunosensor for label-free detection of Campylobacter jejuni in diarrhea patients' stool based on O-carboxymethylchitosan surface modified Fe3O4 nanoparticles

• Published in: Biosensors & bioelectronics Vol. 25; no. 5; pp. 1204 - 1211
• Main Authors: Huang, Jinlin, Yang, Gongjun, Meng, Wenjing, Wu, Liping, Zhu, Aiping, Jiao, Xin'an
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier 15.01.2010
• Subjects: Animals; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensors; Biotechnology; Campylobacter jejuni - isolation & purification; Diarrhea - diagnosis; Diarrhea - microbiology; Electric Impedance; Electrochemistry - instrumentation; Equipment Design; Equipment Failure Analysis; Feces - microbiology; Ferric Compounds - chemistry; Fundamental and applied biological sciences. Psychology; Humans; Immunoassay - instrumentation; Methods. Procedures. Technologies; Nanoparticles - chemistry; Reproducibility of Results; Sensitivity and Specificity; Surface Properties; Various methods and equipments; Electrochemicalimpedance spectroscopy; Nanoparticle; Campylobacteraceae; Diarrhea; Immunosensor; Campylobacter jejuni; O; Spectrometry; Campylobacterjejuni; nanoparticles; Biosensor; O-Carboxymethylchitosan surface modified; Electrochemistry; Bacteria; Feces; Detection; Fe
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2009.10.036

A novel electrochemical impedimetric immunosensor based on O-carboxymethylchitosan surface modified Fe(3)O(4) nanoparticles (denoted as OCMCS-Fe(3)O(4) nanoparticles) was developed for rapid detection of Campylobacter jejuni, which is becoming the most common cause of gastroenteritis in developed countries and raising major public health concerns worldwide. In the present study, anti-FlaA monoclonal antibodies 2D12 (denoted as 2D12McAbs) were immobilized on OCMCS-Fe(3)O(4) nanoparticles. The detection was performed by measuring relative change in impedance before and after 2D12McAbs-Campylobacter jejuni reaction with the technique of electrochemical impedance spectroscopy. Under the optimized conditions, the relative change in impedance was proportional to the logarithmic value of Campylobacter jejuni concentrations in the range of 1.0x10(3) to 1.0x10(7) CFU/mL (r=0.991). The advantages of the OCMCS-Fe(3)O(4) nanoparticle-based immunosensor are simplicity of use, fast response, wide linear range, acceptable reproducibility and long stability. Moreover, the immunosensor could be regenerated by being treated with glycine-HCl buffer solution (pH 2.8). We demonstrate the convenient application of the novel immunosensor for the detection of Campylobacter jejuni in diarrhea patients' stool samples.