Evaluation of soluble CD200 levels in type 2 diabetic foot and nephropathic patients: association with disease activity

• Published in: Medical science monitor Vol. 20; pp. 1078 - 1081
• Main Authors: Arik, Hasan Onur, Yalcin, Arzu Didem, Celik, Betul, Seyman, Derya, Tetik, Gulsum, Gursoy, Bensu, Kose, Sukran, Gumuslu, Saadet
• Format: Journal Article
• Language: English
• Published: United States International Scientific Literature, Inc 26.06.2014
• Subjects: Adult; Aged; Aged, 80 and over; Antigens, CD - blood; Case-Control Studies; Diabetic Foot - blood; Diabetic Foot - pathology; Female; Humans; Kidney Diseases - blood; Kidney Diseases - pathology; Male; Middle Aged; Preliminary Report; Solubility
• ISSN: 1643-3750; 1234-1010; 1643-3750
• DOI: 10.12659/MSM.890517

CD200 (OX-2) is a novel immune-effective molecule, existing in a cell membrane-bound form, as well as in a soluble form in serum (s OX-2), which acts to regulate inflammatory and acquired immune responses. We planned this study to evaluate the sOX-2 levels of type 2 diabetic foot (group B), and compare it with that of healthy controls (group A). The patient group had the following values: DM period: 27.9±10.3 year [mean ±SD], HbA1c: 9.52±2.44% [mean ±SD]. Blood samples for sCD200 measurement were always taken in the morning between 8 and 10 A.M.. The results were reported as means of duplicate measurements. Concentrations of sOX-2 in the serum samples were quantified using an ELISA kit. Serum hs-CRP levels were measured using an hs-CRP assay kit. The sOX-2 level in group B was 173.8±3.1 and in group A was 70.52±1.2 [p<0.0001). In subgroup analysis of T2DM-DFI patients, we noticed that sOX-2 levels were higher in WGS (Wagner grading system) I and II patients than in WGS III and IV patients. The HbA1c, BUN, creatinine, hs-CRP levels, and sedimentation rates were higher in the patient group (p<0.0001, p<0.001, p<0.001, p<0.005, and p<0.0001, respectively). We suggest that there are vascular, immunologic, and neurologic components in DFI, whereas autoimmune diseases and inflammatory skin disorders have only an immunologic component. This is possibly evidence of a pro-inflammatory effect seen in DFI as a vascular complication.