Preparation and characterisation of organic UV filters based on combined PHB/liposomes with natural phenolic compounds

• Published in: Journal of biotechnology Vol. 324; p. 100021
• Main Authors: Pavelkova, Renata, Matouskova, Petra, Hoova, Julie, Porizka, Jaromir, Marova, Ivana
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.01.2020
• Subjects: Coffea arabica; Cytotoxicity; Nanoparticles; PHB-liposomes; Stability; UV protection; Nanoparticles; UV protection; Cytotoxicity; Stability; Coffea arabica; PHB-liposomes
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/j.btecx.2020.100021

[Display omitted] •New UV protecting systems based on liposomes/PHB with encapsulated extracts of green and roasted coffee were fabricated and characterized.•Incorporation of 20 % PHB into liposomes led to preserving their size and colloidal stability, to reduce inflammation and to enhance UV-protective effect.•Addition of 20 % PHB into liposomes led to increased stability during long-term storage and to stabilized values of Sun Protection Factor (SPF).•Cytotoxicity studies showed that new combined PHB liposome particles with coffee extracts were not found as toxic for HaCaT cells and in LDH test.•Liposomes/PHB could be used as active carriers both for hydrophobic and hydrophilic active components, combined with PHB UV-protective effect. In this study preparation and characterization of new UV-protecting systems based on liposomes/polyhydroxybutyrate (PHB) with encapsulated coffee extracts are presented. Green and roasted coffee extracts with high phenolics content, high antioxidant activity and sun protection factor (SPF) value 40–50 were used as model organic UV filters and encapsulated into liposomes and PHB-liposomes. Particle size and colloid stability was observed by dynamic light scattering and zeta-potential. Toxicity of particles was tested by MTT and LDH assay on HaCaT cell line. All prepared samples showed moderate or high encapsulation efficiency. Addition of PHB up to 50 % of lecithin led to increased size and stability. As optimal addition of 20 % PHB into liposome particles was found leading to optimum size and processing of particles, to high UV-protective effect as well as to increased colloid stability and SPF value during long-term storage. Significant differences in cell viability were found in cytotoxicity studies after exposure of keratinocytes to liposomes with different PHB content. Newly fabricated PHB-liposome particles with coffee extract were not found as toxic for HaCaT cells and in LDH test up to 12 %. These particles can act as active carriers for organic sunscreen components in combination with UV-protective effect of PHB.