T-2 toxin immunotoxicity on human B and T lymphoid cell lines
T-2 toxin belongs to a group of mycotoxins synthesized by Fusarium fungi that are widely encountered as natural contaminants in cereals. Human lymphoid cell lines of T (MOLT-4) or B (IM-9) lineage were used to characterize the cytotoxic effects mediated by T-2 at different concentrations (0.1 pg/ml...
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Published in | Toxicology (Amsterdam) Vol. 210; no. 1; pp. 81 - 91 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Shannon
Elsevier Ireland Ltd
15.05.2005
Amsterdam Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | T-2 toxin belongs to a group of mycotoxins synthesized by
Fusarium fungi that are widely encountered as natural contaminants in cereals. Human lymphoid cell lines of T (MOLT-4) or B (IM-9) lineage were used to characterize the cytotoxic effects mediated by T-2 at different concentrations (0.1
pg/ml to 1
μg/ml). After 24
h, membrane damage was observed by Trypan blue dye exclusion in IM-9 cells with a 50% cytotoxic concentration (CC
50) of 0.2
ng/ml, whereas CC
50 for MOLT-4 cells was 0.6
μg/ml (gmicro). At a T-2 concentration of 0.01
μg/ml, apoptosis was seen in MOLT-4 cells by Annexin V binding as early as after 4
h. T-2 toxin determined sustained (48
h) immunosuppression on both cell lines, as evaluated by BrdU and MTT assays. Cytotoxicity appeared to be due to early apoptosis in MOLT-4 cells, as indicated by increased Annexin V binding and activation of caspase-3, and to direct cell membrane damage in IM-9 cells. |
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ISSN: | 0300-483X 1879-3185 |
DOI: | 10.1016/j.tox.2005.01.007 |