Prevalence and antibiotic resistance of Listeria monocytogenes in camel meat

• Published in: Bioscience reports Vol. 40; no. 6
• Main Authors: Yehia, Hany M, Elkhadragy, Manal F, Aljahani, Amani H, Alarjani, Khaloud M
• Format: Journal Article
• Language: English
• Published: England Portland Press Ltd 26.06.2020
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Bacterial Toxins - genetics; Biophysics; Camelus - microbiology; DNA, Bacterial - genetics; Drug Resistance, Multiple, Bacterial; Food Microbiology; Foodborne Diseases - microbiology; Foodborne Diseases - prevention & control; Heat-Shock Proteins - genetics; Hemolysin Proteins - genetics; Listeria monocytogenes - drug effects; Listeria monocytogenes - genetics; Listeria monocytogenes - isolation & purification; Listeriosis - microbiology; Listeriosis - prevention & control; Listeriosis - transmission; Meat - microbiology; Microbial Sensitivity Tests; Molecular Bases of Health & Disease; Neuroscience; Listeria monocytogenes; raw camel meat; antimicrobial resistance; listeriolysin O (hly) gene
• ISSN: 0144-8463; 1573-4935
• DOI: 10.1042/BSR20201062

In the present study, a total of 50 raw camel meat samples were analyzed for the presence of Listeria monocytogenes. The isolates were characterized via morphological and culture analyses; identification of isolates was confirmed by polymerase chain reaction (PCR) and sequencing of the listeriolysin O gene. The API Listeria system was used for further chemical identification and verification of the strains. L. monocytogenes was identified in eight raw camel meat samples, which was the highest incidence (16%) of contamination, followed by L. seeligeri 3(6%), L. innocua and L. welshimeri 2 (2% each), and L. grayi 1 (1%). According to Basic Local Alignment Search Tool (BLAST) analysis, isolated strains that were positive for the listeriolysin O gene were >99% similar to the published database sequences for L. monocytogenes strain LM850658 (sequence ID: CP009242.1). We studied the antibiotic resistance profile of the L. monocytogenes strains with common antibiotics used to treat human listeriosis and demonstrated that almost all strains tested were susceptible to the antibiotics.