Molecular characterization of the Rpv3 locus towards the development of KASP markers for downy mildew resistance in grapevine (Vitis spp.)
Plasmopara viticola is the oomycete that causes downy mildew in grapevines. Varying levels of resistance to P. viticola across grape cultivars allowed quantitative trait loci to be identified. The Rpv3 locus is located at chromosome 18, in a region enriched in TIR-NBS-LRR genes, and the phenotype as...
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Published in | Euphytica Vol. 218; no. 1 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
2022
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Plasmopara viticola
is the oomycete that causes downy mildew in grapevines. Varying levels of resistance to
P. viticola
across grape cultivars allowed quantitative trait loci to be identified. The
Rpv3
locus is located at chromosome 18, in a region enriched in TIR-NBS-LRR genes, and the phenotype associated is a high hypersensitive response. In this work, we aimed to identify candidate genes associated with resistance to downy mildew on the
Rpv3
locus and to evaluate their transcriptional profiles in a susceptible and a resistant grapevine cultivar after challenging with
P. viticola
. Candidate genes were identified by in silico functional enrichment tests. Many predicted genes associated with resistance to diseases were found at the
Rpv3
locus. In total, seventeen genes were evaluated by RT-qPCR. Differences in the steady-state expression of these genes were observed between the two cultivars. Four genes were found to be expressed only in Villard Blanc, suggesting their association to the hypersensitivity reaction. Aiming to assist marker assisted-selection for downy mildew resistance, we show the efficient use of a set of SSR markers. Furthermore, from on a set of forty-one
Rpv3
-located SNPs, whose segregation was tested in the populations studied, the two segregating markers, Rpv3_15 and Rpv3_33, were considered efficient for downy mildew resistance identification. This study presents a genomic characterization of the
Rpv3
locus, confirms its involvement in resistance against
P. viticola
infection and presents promising biotechnological tools for the selection of young resistant individuals. |
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ISSN: | 0014-2336 1573-5060 |
DOI: | 10.1007/s10681-021-02952-3 |