Quantitative changes of bone collagen crosslinks and precursors in vitamin D deficiency

• Published in: Biochemical and biophysical research communications Vol. 47; no. 4; pp. 760 - 765
• Main Authors: Mechanic, Gerald L., Toverud, Svein U., Ramp, Warren K.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 26.05.1972
• Subjects: Aldehydes - metabolism; Amino Alcohols - metabolism; Aminocaproates - metabolism; Animals; Body Weight; Bone and Bones - metabolism; Bone Development; Borohydrides; Chickens; chicks; Chromatography; Collagen - metabolism; Dipeptides - metabolism; Hydroxylysine - metabolism; Male; Oxidation-Reduction; Protein Conformation; Space life sciences; Tibia - metabolism; Tritium; Vitamin D Deficiency - metabolism
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(72)90557-8

The crosslinks from NaB 3H 4-reduced bone collagen of 4-week-old rachitic and normal chicks were compared. The ratio of the reduced crosslink δ,δ′-dihydroxylysinonorleucine (DHLNL) to δ-hydroxylysinonorleucine (HLNL) was significantly increased in vitamin D deficiency. The ratio of ϵ-hydroxynorleucine (HNL) (reduced crosslink precursor of HLNL) to the reduced unknown peak 1 was decreased while that of HLNL to HNL was unaffected. The data indicate that vitamin D affects the quantitative relationships between crosslinks and aldehydic precursors that are present in mineralizing macromolecular matrices.