Novel luminescence assay offers new possibilities for the risk assessment of silica nanoparticles

Recent opinions of the Scientific Committee on Consumer Products (SCCP) of the European Commission emphasize the missing validation of in vitro methodologies for nanomaterials and suggest that a review of the safety of insoluble nanomaterials presently used is required. Therefore the influence of Fl...

Full description

Saved in:
Bibliographic Details
Published inNanotoxicology Vol. 2; no. 4; pp. 243 - 251
Main Authors Wahl, Birgit, Daum, Nicole, Ohrem, Hans-Leonhard, Lehr, Claus-Michael
Format Journal Article
LanguageEnglish
Published Informa UK Ltd 01.01.2008
Taylor & Francis
Subjects
Cytotoxicity
LDH assay
luminescence assay
nanoparticles
silica
Online AccessGet full text

Cover

More Information
Summary:Recent opinions of the Scientific Committee on Consumer Products (SCCP) of the European Commission emphasize the missing validation of in vitro methodologies for nanomaterials and suggest that a review of the safety of insoluble nanomaterials presently used is required. Therefore the influence of Fluospheres® and silica nanoparticles, representing the class of organic and inorganic nanoparticles, respectively, on the lactate dehydrogenase (LDH) assay and the novel luminescence based Vialight® assay was tested. While LDH assay showed strong interactions with the tested silica particles, these problems may be overcome by novel methods like luminescence based assays. These findings suggest that even well characterized assay systems need a careful evaluation of the particle assay interactions when working with nanoparticles. Furthermore, particles based on the same material exhibit different biological properties depending on whether the material is used in micro- or nanometer range.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1743-5390
1743-5404
DOI:10.1080/17435390802513204