A screening assay for detecting CD8 + cell non-cytotoxic anti-HIV responses

• Published in: Journal of immunological methods Vol. 304; no. 1; pp. 137 - 150
• Main Authors: Killian, M. Scott, Ng, Sharon, Mackewicz, Carl E., Levy, Jay A.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.09.2005; Elsevier
• Subjects: Antiviral; Assay; Biological and medical sciences; CD8 + lymphocyte; CD8-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - virology; CNAR; Cytotoxicity, Immunologic; Enzyme-Linked Immunosorbent Assay - methods; Flow Cytometry - methods; Fundamental and applied biological sciences. Psychology; Fundamental immunology; HIV; HIV - immunology; HIV - physiology; HIV Infections - diagnosis; HIV Infections - immunology; HIV Infections - prevention & control; Humans; Immune response; Innate; Killer Cells, Natural - immunology; Leukocytes, Mononuclear - immunology; Leukocytes, Mononuclear - virology; Lymphocyte Count - methods; Lymphocyte Depletion; Mass Screening - methods; Microbiology; Molecular immunology; Techniques; Techniques used in virology; Virology; Virus Replication - physiology; Assay; CNAR; Immune response; HIV; CD8 + lymphocyte; Antiviral; Innate; Retroviridae; Lentivirus; Immunological method; Virus; CD8+ lymphocyte; Human immunodeficiency virus; Detection; Lymphocyte
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/j.jim.2005.07.016

The rate of HIV-1 disease progression is influenced by several factors that include pathogen and host genetic variations and the quality of antiviral immune responses. The CD8 + cell non-cytotoxic antiviral response (CNAR) substantially suppresses HIV replication in CD4 + cells and is positively associated with an asymptomatic clinical state. Traditionally, the measurement of CNAR has required several culture procedures and costly reagents. Here we report the development and validation of a screening assay for detection of CNAR that accurately identifies individuals benefiting from this response. Use of the CNAR screening assay should facilitate the evaluation of this important immune parameter in studies of HIV pathogenesis, resistance to infection, and vaccine development.