Primary structure and expression of a gene encoding the cytosolic ribosomal protein S4 from potato

• Published in: Biochimica et biophysica acta Vol. 1218; no. 3; pp. 435 - 438
• Main Authors: Braun, Hans-Peter, Emmermann, Michael, Mentzel, Helga, Schmitz, Udo Klaus
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 02.08.1994
• Subjects: Amino Acid Sequence; amino acid sequences; Animals; Base Sequence; cytosol; Cytosol - metabolism; embl/x76651; genbank/x76651; genes; Genes, Plant; Humans; Macromolecular Substances; Molecular Sequence Data; nucleotide sequences; Potato; proteins; Rats; Ribosomal protein S4; Ribosomal Proteins - biosynthesis; Ribosomal Proteins - genetics; ribosomes; Saccharomyces cerevisiae - genetics; Sequence Homology, Amino Acid; Solanum tuberosum; Solanum tuberosum - genetics; Solanum tuberosum - metabolism; Tissue specificity; Transcription; transcription (genetics); Amino acid sequence; Tissue specificity; Potato; Ribosomal protein S4; Transcription
• ISSN: 0167-4781; 0006-3002; 1879-2634; 1878-2434
• DOI: 10.1016/0167-4781(94)90200-3

The primary structure of a cDNA clone encoding the S4 protein from the small subunit of 80S ribosomes from potato was determined. Cytosolic ribosomal protein S4 is hydrophilic and has a prevalence for positively charged residues. In potato it is 264 amino acids long and contains a putative nuclear targeting signal close to the N-terminus. Having 65–69% identical amino acids cytosolic ribosomal protein S4 from mammals, fungi and plants belongs to the highly conserved proteins. The S4 gene is transcribed in all potato tissues analysed and has a relatively high expression level in comparison to nuclear genes encoding mitochondrial proteins.