Gold Nanoparticle Based Electrochemical Immunosensor for Detection of T3 Hormone

We report a nanoparticles based electrochemical immunosensor to detect and quantify triiodothyronine (T3) hormone. Immunosensor developed using gold nanoparticles and anti-T3 antibody, was employed for quantification of T3 antigen using cyclic voltammetry (CV) and differential pulse voltammetry (DPV...

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Bibliographic Details
Published inJournal of nanoscience and nanotechnology Vol. 20; no. 10; p. 6057
Main Authors Saxena, Rahul, Fouad, H, Srivastava, Sudha
Format Journal Article
LanguageEnglish
Published United States 01.10.2020
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Summary:We report a nanoparticles based electrochemical immunosensor to detect and quantify triiodothyronine (T3) hormone. Immunosensor developed using gold nanoparticles and anti-T3 antibody, was employed for quantification of T3 antigen using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) technique. The electrochemical response of the developed immunosensor correlates well with the amount of antigen present in the sample. With increase in antigen concentration the immunocomplex formation on electrode surface increases and hence redox current decreases. The immunosensor shows a lower limit of detection of 1 pg/mL and dynamic range from 1 to 500 pg/mL. Sensitivity of the immunosensor was found to be 29.81 A/pg/mL/cm².
ISSN:1533-4899
DOI:10.1166/jnn.2020.18561