Tailoring the in vitro characteristics of poly(vinyl alcohol)-nanohydroxyapatite composite scaffolds for bone tissue engineering
Poly(vinyl alcohol) reinforced with nanohydroxyapatite (PVA-nHA) composite scaffolds were developed by varying the nHA (1%, 2%, 3%, 4%, and 5%, w/v) composition in the PVA matrix by solvent casting technique. The developed composite scaffolds were characterized using scanning electron microscopy (SE...
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Published in | Journal of polymer engineering Vol. 36; no. 8; pp. 771 - 784 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
De Gruyter
01.10.2016
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Subjects | |
Online Access | Get full text |
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Summary: | Poly(vinyl alcohol) reinforced with nanohydroxyapatite (PVA-nHA) composite scaffolds were developed by varying the nHA (1%, 2%, 3%, 4%, and 5%, w/v) composition in the PVA matrix by solvent casting technique. The developed composite scaffolds were characterized using scanning electron microscopy (SEM), X-ray powder diffraction (XRD), attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, and contact angle measurement. The stability of the composite scaffolds in physiological environment was evaluated by swelling and degradation studies. Further, these composite scaffolds were tested for
bioactivity, hemolysis, biocompatibility, and mechanical strength. SEM micrographs showed a homogenous distribution of nHA (3%, w/v) in the PVA matrix. XRD and ATR-FTIR analysis confirmed no phase contamination and the existence of the chemical bond between PVA-nHA at approximately 2474 cm
. PVA-nHA composite scaffolds with 3% (w/v) concentration of nHA showed nominal swelling and degradation behavior with good mechanical strength. The mechanical strength and degradation properties of the scaffold above 3% (w/v) of nHA was found to deteriorate, which is due to the agglomeration of nHA. The
bioactivity and hemolysis studies showed improved apatite formation and hemocompatibility of the developed scaffolds.
cell adhesion, proliferation, alkaline phosphatase activity, and Alizarin red S staining confirmed the biocompatibility of the composite scaffolds. |
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ISSN: | 0334-6447 2191-0340 |
DOI: | 10.1515/polyeng-2015-0252 |