LncRNA DANCR promotes migration and invasion through suppression of lncRNA-LET in gastric cancer cells

• Published in: Bioscience reports Vol. 37; no. 6
• Main Authors: Mao, Zhengqiang, Li, Hang, Du, Botao, Cui, Kai, Xing, Yuguang, Zhao, Xiangyu, Zai, Shoufeng
• Format: Journal Article
• Language: English
• Published: England Portland Press Ltd 22.12.2017
• Subjects: Aged; Cell Line, Tumor; Cell Movement; Cell Proliferation; Enhancer of Zeste Homolog 2 Protein - genetics; Enhancer of Zeste Homolog 2 Protein - metabolism; Epigenesis, Genetic; Gene Expression Regulation, Neoplastic; Histone Deacetylases - genetics; Histone Deacetylases - metabolism; Humans; Lymphatic Metastasis; Male; Middle Aged; Neoplasm Invasiveness; Neoplasm Staging; RNA, Long Noncoding - antagonists & inhibitors; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; Signal Transduction; Stomach Neoplasms - diagnosis; Stomach Neoplasms - genetics; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; lncRNA-LET; HDAC3; DANCR; EZH2
• ISSN: 0144-8463; 1573-4935
• DOI: 10.1042/BSR20171070

Gastric cancer (GC) is one of the most prevalent gastrointestinal malignancies. Long noncoding RNA (lncRNA) DANCR is a newly identified oncogenic lncRNA. However, the functional role and underlying molecular mechanisms of DANCR involved in GC progress remain unclear. In the present study, we investigated the biological function and underlying mechanisms of DANCR in GC cell migration and invasion. The results showed that knockdown of DANCR inhibited migration and invasion of GC cells, whereas overexpression of DANCR showed the opposite effect. Further investigation demonstrated that lncRNA-LET was a target gene of DANCR. In addition, high DANCR and low lncRNA-LET were significantly correlated with lymph node metastasis and late clinical stage. DANCR associated with EZH2 and HDAC3 to epigenetically silence lncRNA-LET and then regulated GC migration and invasion. Taken together, these findings indicate an important role for DANCR-lncRNA-LET axis in GC cell migration and invasion, and reveal a novel epigenetic mechanism for lncRNA-LET silencing.