Partial DNA sequencing of Douglas-fir cDNAs used for RFLP mapping

• Published in: Theoretical and applied genetics Vol. 97; no. 5/6; pp. 771 - 776
• Main Authors: Jermstad, K.D, Bassoni, D.L, Kinlaw, C.S, Neale, D.B
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.10.1998; Berlin Springer Nature B.V
• Subjects: Antisense DNA; Biological and medical sciences; Chromosome mapping; Classical genetics, quantitative genetics, hybrids; complementary DNA; DNA probes; DNA sequencing; Douglas fir; expressed sequence tags; Fundamental and applied biological sciences. Psychology; Genes; Genetic aspects; genetic markers; Genetics of eukaryotes. Biological and molecular evolution; identification; Identification and classification; Microbiology; molecular sequence data; nucleotide sequences; Nucleotide sequencing; plant proteins; Pseudotsuga menziesii; Pteridophyta, spermatophyta; restriction fragment length polymorphism; Vegetals; United States; Genetic mapping; Nucleotide sequence; Chromosome; Homology; Pseudotsuga menziesii; Restriction fragment length polymorphism; Complementary DNA; Gene; Gymnospermae; Softwood forest tree; Coniferales; Spermatophyta; Molecular probe
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220050954

DNA sequences from 87 Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) cDNA RFLP probes were determined. Sequences were submitted to the GenBank dbEST database and searched for similarity against nucleotide and protein databases using the BLASTn and BLASTx programs. Twenty-one sequences (24%) were assigned putative functions; 18 of which were from plant species. Six sequences aligned with conifer genes, including genes from Douglas-fir. Similarities among the 87 sequences were revealed by analyses with FASTA, suggesting either redundancy or isoforms of the same gene. Assignment of putative functions to anonymous cDNA mapped markers will increase the understanding of structural gene organization of the Douglas-fir genome.