Propofol attenuates monocyte-endothelial adhesion via modulating connexin43 expression in monocytes

• Published in: Life sciences (1973) Vol. 232; p. 116624
• Main Authors: Ji, Haocong, Qiu, Rongzong, Gao, Xiaofeng, Zhang, Rui, Li, Xianlong, Hei, Ziqing, Yuan, Dongdong
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.09.2019; Elsevier BV
• Subjects: 1-Phosphatidylinositol 3-kinase; Adhesion; AKT protein; Anesthesia; Anesthetics; Antioxidants; Arteriosclerosis; Atherosclerosis; Attenuation; Cell activation; Cell adhesion; Cell adhesion & migration; Cell adhesion molecules; Connexin 43; Connexin43; Cyclooxygenase-2; Deoxyribonucleic acid; DNA; Endothelial cells; Gene expression; Intravenous administration; Kinases; LFA-1 antigen; Monocyte chemoattractant protein 1; Monocyte-endothelial adhesion; Monocytes; NF-κB protein; PI3K/AKT/NF-κB signaling pathway; Preconditioning; Propofol; Signal transduction; Signaling; siRNA; Umbilical vein; Vascular diseases; VLA-4 antigen; Zonula occludens-1 protein; PI3K/AKT/NF-κB signaling pathway; Connexin43; Propofol; Monocyte-endothelial adhesion; Atherosclerosis
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2019.116624

Monocyte-endothelial adhesion is considered to be the primary initiator of inflammatory vascular diseases, such as atherosclerosis. Connexin 43 (Cx43) has been reported to play an important part in this process, however, the underlying mechanisms are not fully understood. Intravenous anesthetics, propofol is commonly used in the perioperative period and in the intensive care unit, and considered to have good anti-inflammatory and antioxidant effects. Thus, we speculate that propofol could influence monocyte-endothelial adhesion, and explore whether its possible mechanism is relative with Cx43 expression in U937 monocytes influencing cell adhesion of U937 monocytes to human umbilical vein endothelial cells (HUVEC). Cx43-siRNAs or pc-DNA-Cx43 were used to alter Cx43 expression in U937 monocytes. Propofol was given as pretreatments to U937 monocytes. Then, cell adhesion, ZO-1, LFA-1, VLA-4, COX and MCP-1 were determined. PI3K/AKT/NF-κB signaling pathway was explored to clarify the possible mechanism. Alternation of Cx43 expression affects cell adhesion and adhesion molecules significantly, such as ZO-1, LFA-1, VLA-4, COX-2 and MCP-1, the mechanism of which is relative with Cx43 influencing the activation of PI3K/AKT/NF-κB signaling pathway. Preconditioning with propofol at its clinically relevant anesthesia concentration attenuates cell adhesion. Propofol not only decreases Cx43 expression in U937 monocytes, but also depresses the activation of PI3K/AKT/NF-κB signaling pathway. Modulation Cx43 expression in U937 monocytes could affect cell adhesion via regulating the activation of PI3K/AKT/NF-κB signaling pathway. Propofol attenuates cell adhesion via inhibiting Cx43 and its downstream signaling pathway of PI3K/AKT/NF-κB.