THE DETERMINATION OF ANTIBODY TO GROUP A STREPTOCOCCAL POLYSACCHARIDE IN HUMAN SERA BY HEMAGGLUTINATION

A hemagglutination method employing tanned human O erythrocytes sensitized with purified Group A streptococcal polysaccharide has been developed to measure A polysaccharide antibody in antistreptococcal rabbit and human sera. The reaction of sensitized RBC with known Group A streptococcal rabbit ant...

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Bibliographic Details
Published inThe Journal of experimental medicine Vol. 121; no. 5; pp. 793 - 806
Main Authors SCHMIDT, W C, MOORE, D J
Format Journal Article
LanguageEnglish
Published United States The Rockefeller University Press 01.05.1965
Subjects
Animals
Antibodies
Antigens
Carrier State
Child
Chromatography
Hemagglutination
Hemagglutination Inhibition Tests
Hemagglutination Tests
Humans
Immune Sera
Immunoglobulins
Nephritis
Old Medline
Polysaccharides, Bacterial
Rabbits
Rheumatic Fever
Sepsis
Streptococcal Infections
Streptococcus
Streptococcus pyogenes
ANTIBODIES
HEMAGGLUTINATION INHIBITION TESTS
EXPERIMENTAL LAB STUDY
HEMAGGLUTINATION
CHROMATOGRAPHY
CHILD
STREPTOCOCCAL INFECTIONS
STREPTOCOCCUS
POLYSACCHARIDES, BACTERIAL
RABBITS
NEPHRITIS
CARRIER STATE
SEPTICEMIA
RHEUMATIC FEVER
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Summary:A hemagglutination method employing tanned human O erythrocytes sensitized with purified Group A streptococcal polysaccharide has been developed to measure A polysaccharide antibody in antistreptococcal rabbit and human sera. The reaction of sensitized RBC with known Group A streptococcal rabbit antisera, inhibition of hemagglutination with A polysaccharide, and abolition of hemagglutination and hemagglutination-inhibition by exposure of polysaccharide antigen to A enzyme, which destroys the specific immunologic determinant of A carbohydrate, were utilized to demonstrate the specificity of this hemagglutinating system. The results of A polysaccharide antibody determinations on sera from normal persons, patients convalescent from streptococcal infection, and patients with non-suppurative streptococcal sequelae are presented.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0022-1007
1540-9538
DOI:10.1084/jem.121.5.793