Electron spin echo studies on chloroplasts. Spectral characteristics of electron transport components and light-induced transients

• Published in: Biochimica et biophysica acta Vol. 590; no. 1; pp. 74 - 88
• Main Authors: Nishi, N, Hoff, A.J, Waals, J.H. van der
• Format: Journal Article
• Language: English
• Published: Netherlands 07.03.1980
• Subjects: Chloroplasts - analysis; Chloroplasts - drug effects; Diuron - pharmacology; Electron Spin Resonance Spectroscopy; Electron Transport; Ferredoxins - analysis; Light; Plants - analysis; Plastocyanin - analysis; spinach; Time Factors
• ISSN: 0006-3002; 1878-2434
• DOI: 10.1016/0005-2728(80)90147-4

Electron-spin resonance echoes are used to study the complex overlapping ESR spectra of whole chloroplasts. By varying the repetition rate of the microwave pulse sequence, delay time, and pulse width, signals with different longitudinal and transverse relaxation times were extracted. We have identified the echo signals due to plastocyanin and ferredoxins. In addition, we have found a strong signal at g = 4.3, that possibly arises from distorted cytochrome, and weak signals in the region g = 6-9. The strong echo signal at g = 2.0047 (Signal II), is made up of at least three "dark" components that differ in their relaxation times. Upon illumination at 1.2 K several of the echo signals including Signal II show reversible light-induced components. The kinetics of these transients depend on the addition of 3(3,4-dichlorophenyl)-1,1-dimethyl urea. Part of the transients are believed to arise from cyclic electron flow around Photosystem I.