Differential expression of miRNA in Carassius auratus gibelio in response to cyprinid herpesvirus 2 infection

• Published in: Developmental and comparative immunology Vol. 82; pp. 1 - 6
• Main Authors: Lu, Jianfei, Xu, Dan, Shen, Zhaoyuan, Lu, Liqun
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.05.2018; Elsevier Science Ltd
• Subjects: Apoptosis; Biological activity; Carassius auratus gibelio; Cyprinid Herpesvirus-2; Gene expression; Herpes viruses; Immune regulation; Immune response; Immune system; MAP kinase; MicroRNAs; miRNA; Molecular chains; Molecular modelling; Next-generation sequencing; Pathogenicity; Pathogens; Polymerase chain reaction; Post-transcription; Regulatory mechanisms (biology); Reverse transcription; Signal transduction; Signaling; Viruses; Cyprinid Herpesvirus-2; miRNA; Immune regulation; Carassius auratus gibelio
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2017.12.027

miRNAs (microRNAs), a small endogenous non-coding RNAs, play crucial roles in post-transcriptional regulator of genes expression in various biological processes. Cyprinid Herpesvirus-2 (CyHV-2) is a highly pathogenic member of the alloherpesviridae that causes acute mass mortalities in populations of Carassius auratus gibelio and Carassius auratus auratus. However, the molecular mechanisms underlying the pathogenicity of CyHV-2 have not been fully determined. Here, miRNA expression profiles were identified via high-throughput sequencing in the kidney of Carassius auratus gibelio infected or uninfected with CyHV-2. The results showed that a total number of 840 known miRNAs and 48 putative novel miRNAs were identified. Then we compared the expression patterns of miRNAs in the two groups, 23 miRNAs were significantly differentially expressed between the uninfected and infected groups. Further, the expressions of 23 miRNAs were validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR), the results showed that the expression patterns were basically the same with the sequencing. Prediction of targets of differentially expressed miRNAs revealed that the miRNAs participated in the regulation of multiple immune-related signaling pathways, including Chemokine signaling pathway, Apoptosis, Jak-STAT signaling pathway and MAPK signaling pathway. Taken together, these data provide insight into the regulatory mechanisms of miRNA and highlight the function of miRNA in the regulation of the immune response during the interaction between host and virus pathogens. •The molecular mechanisms of CyHV-2 pathogenicity have not been elucidated.•We screened miRNAs in C. auratus gibelio kidneys following CyHV-2 infection.•High-throughput sequencing revealed 840 known miRNAs and 48 putative novel miRNAs.•After CyHV-2 infection, 23 miRNAs were significantly differentially expressed.•KEGG analysis showed that they are involved in immune-related signaling.