Platelet aggregation is affected by nitrosothiols in patients with chronic hepatitis: in vivo and in vitro studies

• Published in: World journal of gastroenterology : WJG Vol. 13; no. 27; pp. 3677 - 3683
• Main Authors: Federico, A, Filippelli, A, Falciani, M, Tuccillo, C, Tiso, A, Floreani, A, Naccarato, R, Rossi, F, Del Vecchio Blanco, C, Loguercio, C
• Format: Journal Article
• Language: English
• Published: United States Inter-University Research Centre on Foods, Nutrition and Gastrointestinal Tract, Gastroenterology School, 2nd University of Naples, Italy%Department of Experimental Medicine, 2nd University of Naples, Italy%Department of Surgical and Gastroenterological Sciences, University of Padua, Italy 21.07.2007; Baishideng Publishing Group Co., Limited
• Subjects: Adult; Aged; Aged, 80 and over; Aldehydes - blood; Biomarkers - blood; Blood Platelets - metabolism; Case-Control Studies; Cysteine - blood; Female; Glutathione - blood; Hepatitis C, Chronic - blood; Hepatitis C, Chronic - complications; Hepatitis C, Chronic - metabolism; Humans; Interleukin-6 - blood; Male; Malondialdehyde - blood; Middle Aged; Nitric Oxide - metabolism; Nitrites - blood; Platelet Aggregation; Platelet Aggregation Inhibitors - metabolism; Platelet Count; Platelet Function Tests; S-Nitrosoglutathione - metabolism; S-Nitrosothiols - blood; S-Nitrosothiols - metabolism; Thrombocytopenia - blood; Thrombocytopenia - metabolism; Thrombocytopenia - virology; Tumor Necrosis Factor-alpha - blood; Viral Hepatitis; Oxidative stress; Anti-aggregant; Hepatitis C; Function of platelets; Liver disease
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v13.i27.3677

To investigate the relationship among the number of platelets and plasma levels of S-nitrosothiols (S-NO), nitrite, total non-protein SH (NPSH), glutathione (GSH), cysteine (CYS), malondialdehyde (MDA), 4-hydroxininenal (4HNE), tumor necrosis factor-alpha (TNFalpha) and interleukin (IL)-6 in patients with chronic hepatitis C (CH). In vitro the aggregation of platelets derived from controls and CH patients was evaluated before and after the addition of adenosine diphosphate (ADP) and collagen, both in basal conditions and after incubation with nitrosoglutathione (GSNO). In vivo, S-NO plasma levels increased significantly in CH patients and they were significantly directly correlated with platelet numbers. Patients with platelet counts < 150000/microL, had a smaller increase in S-NO, lower levels of GSH, CYS, NPSH, TNFalpha, and IL-6, and higher levels of nitrite, MDA, and 4-HNE relative to those of patients with platelet counts > 150000/microL. In vitro, the ADP and collagen aggregation time was increased in platelets from patients and not from controls; in addition, platelets from CH patients but not from controls also showed a latency time after exposure to collagen. The incubation of platelets with GSNO improved the percentage aggregation and abolished the latency time.