Structure of the 19 S RNA transcript encoded by the cauliflower mosaic virus genome

• Published in: Virology (New York, N.Y.) Vol. 111; no. 2; pp. 377 - 385
• Main Authors: Odell, Joan T., Keith Dudley, R., Howell, Stephen H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.1981
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/0042-6822(81)90341-X

Turnip leaves infected with cauliflower mosaic virus accumulate several virus-derived RNA species. These include a large transcript (>25 S) and a 19 S transcript. R-looping and diazo-cellulose filter hybridization techniques show that the 19 S RNA is derived from a contiguous segment of the CaMV genome which includes EcoR1-b and -d DNA fragments. This region encodes the synthesis of a nonvirion protein called P66. The 3′ end of the 19 S transcript is polyadenylated and terminates near the single strand break in the α-strand of the genome. The 5′ end of the transcript starts in or near the EcoR1-d fragment, near position zero on the map described by Hull and Howell (1978).