Induction of innate immune response in whiteleg shrimp (Litopenaeus vannamei) embryos

• Published in: Developmental and comparative immunology Vol. 105; p. 103577
• Main Authors: Alvarez-Lee, Angélica, Martínez-Díaz, Sergio F., Gutiérrez-Rivera, Jesus Neftalí, Lanz-Mendoza, Humberto
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.04.2020; Elsevier Science Ltd
• Subjects: Aquaculture; Bacillus; Bacteria; Bacterial diseases; Decapoda; Disease control; Embryos; Gene expression; Hatching; Heat treatment; Immune priming; Immune response; Immune system; Innate immunity; Invertebrates; Larval development; Litopenaeus vannamei; Marine invertebrates; Shellfish; Shrimp embryo; Training; Vibrio; Waterborne diseases; Litopenaeus vannamei; Shrimp embryo; Immune priming; Bacillus; Vibrio
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2019.103577

The immune response of commercially relevant marine invertebrates has been extensively studied, in search of new disease-control strategies. Immune training is considered a novel approach that could help improve resistance to different pathogens. Here, we stimulated the white shrimp (Litopenaeus vannamei) during embryo development by exposure to heat-killed bacteria and evaluated their effect on hatching, larval development, and the expression of immune-related genes. In addition, we evaluated its impact on the response of shrimp nauplii during a challenge with Vibrio parahaemolyticus. We observed that the percentage of hatching and the resistance to bacterial infection increased due to the treatment of embryos with heat-killed cells of Vibrio and Bacillus. Apparently different stimuli could generate a differential pattern of gene expression, e.g., Vibrio induced a strong effector immune response whereas Bacillus elicited a protective immune profile. In addition, each response was triggered by molecular patterns detected in the environment. The results obtained in this study provide new insights for immune training to improve shrimp farming. •Shrimp embryos respond to a specific stimulus with activation and effectors production of the innate immune system.•Immune response of shrimp embryos was differential depending of kind of microorganism used to stimulate.•Nauplius larvae improve its immunocompetence when was stimulated during embryo development.