Selenium/Zinc-Enriched probiotics improve serum enzyme activity, antioxidant ability, inflammatory factors and related gene expression of Wistar rats inflated under heat stress

• Published in: Life sciences (1973) Vol. 248; pp. 117464 - 10
• Main Authors: Malyar, Rahmani Mohammad, Li, Hu, Liu, Dandan, Abdulrahim, Yassin, Farid, Rawan Ahmad, Gan, Fang, Ali, Waseem, Enayatullah, Hamdard, Banuree, Sayed Attaul Haq, Huang, Kehe, Chen, Xingxiang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.05.2020; Elsevier BV
• Subjects: Alanine; Alanine Transaminase - genetics; Alanine Transaminase - metabolism; Alkaline phosphatase; Alkaline Phosphatase - genetics; Alkaline Phosphatase - metabolism; Ambient temperature; Animals; Antioxidant ability; Antioxidants; Antioxidants - administration & dosage; Aspartate Aminotransferases - genetics; Aspartate Aminotransferases - metabolism; Blood; Blood Urea Nitrogen; Creatine; Creatine Kinase - genetics; Creatine Kinase - metabolism; Creatinine; Creatinine - metabolism; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - metabolism; Cyclooxygenase-2; Data analysis; Diet; Dietary Supplements; Enrichment; Enzymatic activity; Enzyme activity; Enzymes; Gene expression; Gene Expression Regulation - drug effects; Glutathione; Glutathione - metabolism; Glutathione Peroxidase - genetics; Glutathione Peroxidase - metabolism; Heat shock; Heat shock proteins; Heat stress; Heat tolerance; Heat-Shock Response - drug effects; Heat-Shock Response - genetics; HSP70 Heat-Shock Proteins - genetics; HSP70 Heat-Shock Proteins - metabolism; Hsp70 protein; Inflammation; Interleukin 10; Interleukin 2; Interleukin 6; Interleukin 8; Kinases; L-Lactate Dehydrogenase - genetics; L-Lactate Dehydrogenase - metabolism; Lactic acid; Male; Malondialdehyde; Malondialdehyde - metabolism; Nitric Oxide Synthase Type II - genetics; Nitric Oxide Synthase Type II - metabolism; Nitric-oxide synthase; Peroxidase; Probiotics; Probiotics - administration & dosage; Rats; Rats, Wistar; Rodents; Selenium; Selenium - administration & dosage; Selenium/Zinc-Enriched probiotics; Serum enzyme activity; Superoxide dismutase; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Thermal stress; Urea; Wistar rats; Zinc; Zinc - administration & dosage; Heat shock proteins; Antioxidant ability; Wistar rats; Serum enzyme activity; Heat stress; Selenium/Zinc-Enriched probiotics
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2020.117464

The present study was carried out to investigate the influences of Selenium/Zinc-Enriched probiotics (SeZnP) on growth performance, serum enzyme activity, antioxidant capability, inflammatory factors and gene expression associated with Wistar rats inflated under high ambient thermal-stress. Sixty male rates with six-weeks of age were randomly allocated into five groups (12 per group) and fed basal diet (Control), basal diet supplemented with probiotics (P), Zinc-Enriched probiotics (ZnP, 100 mg/L), Selenium-Enriched Probiotics (SeP, 0.3 mg/L) and Selenium/Zinc-Enriched probiotics (SeZnP, 0.3 mg + 100 mg/L). The experiment lasted 30 days. Blood and Tissues samples were taken to investigate serum enzyme activity, antioxidants capability and inflammatory factors by using of commercial kits and antioxidant, heat shock and inflammatory related molecules expressions were determined by qRT-PCR. Data analysis revealed that thermal stress significantly increased the level of Aspartate-aminotransferase, Alanine-aminotransferase, Lactate-dehydrogenase, Creatine-kinase, blood urea nitrogen, Creatinine and Alkaline phosphatase compared to P, ZnP, SeP or SeZnP groups (P < 0.01). However, supplementation of ZnP, SeP, and SeZnP significantly enhanced glutathione content, glutathione-peroxidase & superoxide-dismutase activity, and decreased malondialdehyde content (P < 0.05). Moreover, the concentration of IL-2, IL-6 and IL-8 were significantly increased while IL-10 was significantly decreased (P < 0.05). Furthermore, the expression of GPx1 and SOD1 genes were significantly increased, but COX-2, iNOS, HSP70 and 90 mRNA levels were significantly decreased (P < 0.05). Finally, the highest influence of the mentioned parameters was observed in SeZnP supplemented group. Our study suggests that SeZnP supplementation serves as possible and best nutritive than ZnP or SeP for Wistar rats raising under high ambient temperature. •Selenium/Zinc-Enriched probiotics increased glutathione content, glutathione-peroxidase & superoxide-dismutase activities and decreased malondialdehyde content in serum of Wistar rats.•Selenium/Zinc-Enriched probiotics decreased the level of aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, creatine kinase, blood urea nitrogen, creatinine, and alkaline phosphatase in serum of Wistar rats.•Selenium/Zinc-Enriched probiotics increased GPx1, SOD1 genes and decreased Hsp90, Hsp70, COX-2 and iNOS genes.•Selenium/Zinc-Enriched probiotics supplementation serves as possible nutritive for Wistar rats raised under heat stress.