A circular cell culture system using microalgae and mammalian myoblasts for the production of sustainable cultured meat

For sustainable production of cultured meat, we propose a novel circular cell culture (CCC) system in which microalgae are used as nutrient supply for the mammalian cell culture and as a waste-medium recycler. Chlorococcum littorale , RL34 hepatocytes, and C2C12 myoblasts were used as cell sources f...

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Published inArchives of microbiology Vol. 204; no. 10; p. 615
Main Authors Haraguchi, Yuji, Okamoto, Yuta, Shimizu, Tatsuya
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2022
Springer Nature B.V
Subjects
Algae
Amino acids
Aquatic microorganisms
Archives & records
Biochemistry
Biomedical and Life Sciences
Biotechnology
Carbon dioxide
Cell Biology
Cell culture
Cell growth
Cultured meat
Ecology
Emissions
Energy consumption
Energy costs
Environmental impact
Growth factors
Hepatocytes
Life Sciences
Livestock
Mammals
Meat
Meat production
Microalgae
Microbial Ecology
Microbiology
Muscles
Myoblasts
Nutrients
Original Paper
Sustainable production
Circular cell culture (CCC) system
Microalgae
Sustainable
Media recycling
Serum-free mammalian cell proliferation
Cultured meat
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Summary:For sustainable production of cultured meat, we propose a novel circular cell culture (CCC) system in which microalgae are used as nutrient supply for the mammalian cell culture and as a waste-medium recycler. Chlorococcum littorale , RL34 hepatocytes, and C2C12 myoblasts were used as cell sources for microalgae, growth factor-producing cells, and muscle cells, respectively. In the first cycle, C2C12 cells were amplified 4.0-fold after 48 h of culture in an RL34 cell-conditioned medium. In the second cycle, C2C12 cells were cultured in the C. littorale culture waste medium to which the C. littorale -derived nutrients were added. The proliferation rates of C. littorale and C2C12 and the nutrient extraction efficiency from C. littorale were the same in the first and second cycles. Therefore, this CCC system, which works without additional grain-derived nutrients and animal sera, will help drastically reduce environmental load, resource/energy consumption, and costs in future cultured meat production.
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Communicated by Erko Stackebrandt.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-022-03234-9