Longevity-associated NADH dehydrogenase subunit-2 polymorphism and serum electrolyte levels in middle-aged obese Japanese men

• Published in: Mechanisms of ageing and development Vol. 126; no. 6; pp. 705 - 709
• Main Authors: Kokaze, Akatsuki, Ishikawa, Mamoru, Matsunaga, Naomi, Yoshida, Masao, Makita, Ryuji, Satoh, Masao, Teruya, Koji, Sekiguchi, Kanako, Masuda, Yumi, Harada, Matsuko, Uchida, Yoshiko, Takashima, Yutaka
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.06.2005; Elsevier Science
• Subjects: Amino Acid Substitution - genetics; Asian Continental Ancestry Group; Biological and medical sciences; Catalytic Domain - genetics; Chlorides - blood; Development. Metamorphosis. Moult. Ageing; DNA, Mitochondrial - genetics; Female; Fundamental and applied biological sciences. Psychology; Humans; Japan; Longevity - genetics; Longevity - physiology; Longevity-associated mitochondrial DNA polymorphism; Male; Middle Aged; NADH dehydrogenase; NADH Dehydrogenase - genetics; Polymorphism, Restriction Fragment Length; Serum chloride levels; Serum sodium levels; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Japan; NADH dehydrogenase; Serum sodium levels; Serum chloride levels; Longevity-associated mitochondrial DNA polymorphism; Human; Obesity; Senescence; Enzyme; Nutrition disorder; Mitochondrial DNA; Longevity; Subunit; Vertebrata; Mammalia; Electrolyte; Sodium; Chlorides; Oxidoreductases; Nutritional status; Polymorphism
• ISSN: 0047-6374; 1872-6216
• DOI: 10.1016/j.mad.2005.01.005

Mitochondrial DNA 5178 cytosine/adenine polymorphism, which is also called NADH dehydrogenase subunit-2 237 leucine/methionine (ND2-237 Leu/Met) polymorphism is associated with Japanese longevity. This polymorphism is widely associated with blood pressure, serum lipid levels, hematological parameters, intraocular pressure, and serum protein fraction levels. However, there have been no reports on the association between ND2-237 Leu/Met polymorphism and serum electrolyte levels. To investigate this relationship, we performed an association study in 321 healthy middle-aged Japanese men. Crude data showed that serum sodium levels and serum chloride levels were significantly lower in men with ND2-237 Met than in those with ND2-237 Leu ( P = 0.021 and 0.003, respectively). Cigarette consumption and body mass index were significantly and positively associated with serum chloride levels ( P = 0.002 and 0.008, respectively) and hemoglobin levels were significantly and negatively associated with them ( P = 0.007) in ND2-237 Leu genotypic men. In men with ND2-237 Met, only hemoglobin levels were significantly and negatively associated with serum chloride levels ( P = 0.025). After adjusting for covariates, only in male obese (body mass index ≥ 25) subjects, serum sodium and chloride levels remained significantly lower, and serum calcium levels appeared to be significantly higher in ND2-237 Met than in ND2-237 Leu ( P = 0.013, <0.001, and 0.046, respectively). Longevity-associated NADH dehydrogenase subunit-2 polymorphism may influence serum electrolyte levels in middle-aged obese Japanese men.