The genotype of Toxoplasma gondii strains causing ocular toxoplasmosis in humans in Brazil

• Published in: American journal of ophthalmology Vol. 139; no. 2; pp. 350 - 351
• Main Authors: Vallochi, Adriana Lima, Muccioli, Cristina, Martins, Maria Cristina, Silveira, Cláudio, Belfort, Rubens, Rizzo, Luiz Vicente
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.02.2005; Elsevier
• Subjects: Animals; Antigens, Protozoan - genetics; Biological and medical sciences; Brazil - epidemiology; Chorioretinitis - epidemiology; Chorioretinitis - parasitology; DNA, Protozoan - analysis; Eye Banks; Eye Enucleation; Genotype; Human protozoal diseases; Humans; Infectious diseases; Medical sciences; Parasitic diseases; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Protozoal diseases; Protozoan Proteins - genetics; Toxoplasma - genetics; Toxoplasmosis; Toxoplasmosis, Ocular - epidemiology; Toxoplasmosis, Ocular - parasitology; South America; Brazil; America; Human; Protozoa; Apicomplexa; Protozoal disease; Ocular; Genotype; Parasitosis; Strain; Infection; Eye; Toxoplasma gondii; Toxoplasmosis; Ophthalmology
• ISSN: 0002-9394; 1879-1891
• DOI: 10.1016/j.ajo.2004.07.040

To determine Toxoplasma gondii genotype in scars of retinochoroiditis in humans. Immunopathology analysis. DNA was isolated from retinochoroidal specimens taken from the borders of the scars from eye bank eyes as well as enucleated eyes from uveitis patients with retinochoroidal toxoplasmic scars. The SAG2 region was amplified by polymerase chain reaction and analyzed by restriction fragment length polymorphism. Of the 92 eyes studied, 9 had toxoplasmic scars. All of them, as well as the 2 enucleated eyes, disclosed the presence of type I parasites by the gel electrophoresis analysis of the restriction fragment length polymorphism from SAG2. These results suggest that type I strain seems to be responsible for the ocular infections in the population studied.